Identification of MicroRNAs Differentially Expressed in Lung Carcinoid Subtypes and Progression

Neuroendocrinology
Ida RapaMarco Volante

Abstract

To extensively explore microRNA expression profiles in lung carcinoids in correlation with clinical and pathological features. A PCR-based array was employed in the screening phase to analyze 752 microRNAs in a discovery set of 12 lung carcinoids, including 6 typical (3 with lymph node metastasis) and 6 atypical (3 with lymph node metastasis). The results were validated by means of real-time PCR in 37 carcinoids, including 22 typical (4 with lymph node metastasis) and 15 atypical (7 with lymph node metastasis), and 19 high-grade neuroendocrine carcinomas. Unsupervised cluster analysis segregated the pilot cases into 3 distinct groups. Twenty-four microRNAs were differentially regulated in atypical versus typical carcinoids, and 29 in metastatic versus nonmetastatic cases. Eleven microRNAs were selected for validation. All but 1 were significantly different among lung neuroendocrine tumor histotypes. Moreover, 5 (miR-129-5p, miR-409-3p, miR-409-5p, miR-185 and miR-497) were significantly upregulated in typical compared to atypical carcinoids. MiR-409-3p, miR-409-5p and miR-431-5p were also significantly downregulated in carcinoids metastatic to the lymph nodes. Predictive in silico analysis of specific target genes showed that t...Continue Reading

Citations

May 12, 2016·European Journal of Endocrinology·Joakim Crona, Britt Skogseid
Apr 9, 2017·Endocrine-related Cancer·Maria Chiara ZatelliAnnamaria Colao
Dec 15, 2017·Histopathology·Giulio RossiMauro Papotti
Jul 16, 2017·Endocrine-related Cancer·Annunziata Di DomenicoAurel Perren
Jun 28, 2016·Oncotarget·Gang YangYupei Zhao
Jul 16, 2016·Oncotarget·Mauro CivesFranco Silvestris
Oct 12, 2017·Frontiers in Cell and Developmental Biology·Simone DetassisMichela A Denti
Jan 13, 2021·Biomedicines·Patricia Mondelo-MacíaRoberto Díaz-Peña
Jun 3, 2021·Genes·Lilla KrokkerHenriett Butz
Jul 3, 2021·Non-coding RNA·Alexandra KorotaevaAlexander Karpukhin

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