Identification of microRNAs involved in cold adaptation of Litopenaeus vannamei by high-throughput sequencing

Gene
Pingping HeXiaohan Chen

Abstract

The Litopenaeus vannamei (L. vannamei) is one of the most widely cultured shrimp species in the world, with low temperature being one of the most serious threats to its growth and survival. To examine the potential regulatory mechanism of cold adaptation, we conducted a microRNAs (miRNAs) analysis on the hepatopancreas of L. vannamei under normal temperature 28 °C (M28), cold acclimation 16 °C for 6 days (M16), and recovered under normal temperature (MR). In total 14,754,823, 14,945,246 and 15,880,093 raw reads representing 10,690,259, 8,587,144, and 11,512,941 unique sequences of 18-32 nt length were obtained from the M28, M16 and MR libraries, respectively. After comparing the miRNA sequences with the miRBase database, 68 known mature miRNAs and 47 novel miRNAs were identified. Expression analysis showed that 34 miRNAs were significantly differential expressed in response to cold adaptation. Compared to the M28 library, 21 miRNAs were upregulated and 13 miRNAs were downregulated significantly in the M16 library. After recovery to normal temperature, there are 16 miRNAs upregulated and 15 miRNAs downregulated significantly compared to M28 library. Then, five significantly differential expressed miRNAs under cold acclimation in...Continue Reading

Citations

May 2, 2019·Animal Biotechnology·Yongcai WangGuirong Sun
Dec 4, 2019·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Zhenlu WangLanfen Fan
Mar 7, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Fajun LiAili Wang

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