Identification of microsomal triglyceride transfer protein in intestinal brush-border membrane

Experimental Cell Research
Isabelle SlightE Levy

Abstract

Microsomal triglyceride transfer protein (MTP) is a heterodimeric complex consisting of a unique large 97-kDa protein and the multifunctional 58-kDa protein disulfide isomerase (PDI). It plays an essential role in the assembly of lipoproteins by shuttling lipids between phospholipid membranes. Based on cell fractionation, early studies have suggested the endoplasmic reticulum (ER) as the exclusive site of MTP. Focusing on the plasma membrane in this study, our attempts with immunoelectron microscopy and specific antibodies surprisingly revealed that labeling was not exclusively confined to the microsomes of rat absorptive cells. Immunogold labeling was also detected over the microvillus membrane of enterocytes. Western blot analysis and biochemical activity measurement confirmed MTP protein expression in brush-border membrane vesicles (BBMV) isolated from the intestinal epithelial cells of various species. Furthermore, MTP was coexpressed in microvilli membrane with PDI that is crucial to maintain the structure and activity of the MTP complex. The treatment of Caco-2 cells with nocodazole and colchicine blocked the appearance of MTP in the apical membrane. Similarly, the addition of BMS-197636, a known inhibitor of MTP transfer...Continue Reading

References

Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W StremmelP D Berk
Sep 27, 1973·Biochimica Et Biophysica Acta·J SchmitzR K Crane
Jan 1, 1982·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Bendayan
Jan 1, 1982·The Journal of Cell Biology·D D SabatiniM Adesnik
Jan 1, 1995·Progress in Histochemistry and Cytochemistry·M Bendayan
Sep 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S A Ibrahim, K A Balasubramanian
May 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E LevyE Seidman
Mar 24, 1995·The Journal of Biological Chemistry·H JamilJ R Wetterau
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·H JamilJ R Wetterau
Apr 1, 1997·Biochimica Et Biophysica Acta·J R WetterauH Jamil
Dec 22, 1999·Seminars in Cell & Developmental Biology·R Noiva
Mar 14, 2000·The Journal of Biological Chemistry·G K LeungS G Young
Jun 17, 2000·Biochimica Et Biophysica Acta·D L Daleke, J V Lyles
Oct 14, 2000·Annual Review of Cell and Developmental Biology·K H Downing
Mar 27, 2001·Current Opinion in Lipidology·G S Shelness, J A Sellers
Aug 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jogchum Plat, Ronald P Mensink
Jul 3, 2003·Journal of Lipid Research·Larry L SwiftW Gray Jerome
Oct 21, 2003·Molecular Biology of the Cell·Etienne MorelFrançois Delers

❮ Previous
Next ❯

Citations

Apr 4, 2009·PLoS Pathogens·Stephanie Abromaitis, Richard S Stephens
Jul 1, 2009·The Journal of Cell Biology·Russell E McConnellMatthew J Tyska
Mar 13, 2009·Progress in Lipid Research·Isabelle NiotPhilippe Besnard
Sep 15, 2011·Fundamental & Clinical Pharmacology·François ReichardtJean-Hervé Lignot
Jul 11, 2006·Journal of Lipid Research·Alain Théophile SanéEmile Levy
Oct 15, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Noël PerettiEmile Levy
Jul 20, 2007·The Journal of Biological Chemistry·Peter J MohlerLarry L Swift
Oct 8, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Geneviève MailhotEmile Levy

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.