Identification of milk fat triacylglycerols by capillary supercritical fluid chromatography-atmospheric pressure chemical ionization mass spectrometry

Lipids
P Laakso, P Manninen

Abstract

Identification of milk fat triacylglycerols was accomplished by capillary supercritical fluid chromatography (SFC) combined with atmospheric pressure chemical ionization mass spectrometry [(APCI)MS]. Supercritical carbon dioxide was the carrier fluid in SFC. Ionization was achieved by introducing vapor of ammonia in methanol into the ionization chamber which resulted in the formation of abundant [M + 18]+ and [M - RCCO]+ ions of triaclyglycerols. These ions defined both the molecular weight and the fatty acid constituents of a triacylglycerol, respectively. SFC on a nonpolar stationary phase provided an efficient separation of triacylglycerols according to the combined number of carbon atoms in the acyl chains a molecule. In addition to the identification of the major chromatographic peaks representing molecules with 26-54 acyl carbons, minor peaks representing triacylglycerols with an odd number of acyl carbons were separated and identified. Furthermore, compositional information on partially separated isobaric triacylglycerols, which differed substantially in the chain length of the fatty acyl residues, was achieved within some of the peaks. A new finding of the present study was the formation of abundant [M + 18]+ ions of sa...Continue Reading

References

Sep 1, 1973·Analytical Chemistry·T Murata, S Takahashi
Jul 1, 1993·Journal of Dairy Science·J GrestiJ Bezard

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Citations

Jul 31, 2013·Journal of Chromatography. a·José L BernalLaura Toribio
Mar 28, 2001·Journal of Chromatography. a·T BambaA Kobayashi
Jun 20, 2015·International Journal of Molecular Sciences·Laurent LaboureurDavid Touboul
Dec 22, 2005·Mass Spectrometry Reviews·Philippe A Guy, François Fenaille
Oct 4, 2016·Journal of Separation Science·Paola DonatoLuigi Mondello
Mar 27, 2002·Journal of Dairy Science·Robert G Jensen
Aug 29, 2018·Nutrients·Alexandra D GeorgeDonna T Geddes
May 25, 2021·Journal of Dairy Science·Sara Pacheco-PappenheimHein J F van Valenberg
Nov 20, 2001·Chemical Reviews·R C MurphyJ Hevko

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