Identification of miR-9 as a negative factor of insulin secretion from beta cells

Journal of Physiology and Biochemistry
Dongzhi HuDalu Kong

Abstract

MicroRNA is a novel class of small noncoding RNA that has been implicated in a variety of physiological and pathological processes, including glucose homeostasis and diabetes mellitus. So far, a few studies have reported that miRNAs may be an important regulator in glucose-stimulated insulin secretion (GSIS) pathway. However, the role of miRNAs in this process remains unclear. The levels of miRNAs in mouse islets and MIN6 cells were determined by quantitative RT-PCR. Concentration of insulin was determined by ELISA, and the expression of the target protein was determined with western blot assay. The overexpression and downregulation of miRNAs in MIN6 were conducted using cell transfection methods. And luciferase assay was used to measure the direct interaction between miRNAs and target messenger RNAs 3'UTR. miR-9 was screened out for it was downregulated under the effects of short-term high glucose, while long-term high glucose relatively increased miR-9 expression. The Stxbp1 expression was decreased with the overexpression of miR-9 in MIN6 cells and increased when miR-9 was downregulated. Moreover, it was verified by luciferase assay that miR-9 regulated Stxbp1 gene expression by directly targeting Stxbp1 messenger RNA 3'UTR....Continue Reading

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Citations

Jan 24, 2020·Mini Reviews in Medicinal Chemistry·Srijit DasDennis Kheng Zhou
Sep 24, 2020·International Journal of Molecular Sciences·Adam WłodarskiAgnieszka Śliwińska
Apr 4, 2021·International Journal of Molecular Sciences·Hsiuying Wang

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Methods Mentioned

BETA
transfection
PCR
ELISA

Software Mentioned

miRbase
Targetscan

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