Identification of miR-93 as a suitable miR for normalizing miRNA in plasma of tuberculosis patients

Journal of Cellular and Molecular Medicine
Simone E BarryBernadette M Saunders

Abstract

Tuberculosis (TB) remains a major public health issue. New tests to aid diagnoses and monitor the response to therapy are urgently required. There is growing interest in the use of microRNA (miRNA) profiles as diagnostic, prognostic or predictive markers in a range of clinical and infectious diseases, including Mycobacterium tuberculosis infection, however, challenges exist to accurately normalise miRNA levels in cohorts. This study examined the appropriateness of 12 miRs and RNU6B to normalise circulating plasma miRNA levels in individuals with active TB from 2 different geographical and ethnic regions. Twelve miRs (let-7, miR-16, miR-22, miR-26, miR-93, miR-103, miR-191, miR-192, miR-221, miR-423, miR-425 and miR-451) and RNU6B were selected based on their reported production by lung cells, expression in blood and previous use as a reference miRNA. Expression levels were analysed in the plasma of newly diagnosed TB patients from Australia and China compared with individuals with latent TB infection and healthy volunteers. Analysis with both geNorm and NormFinder software identified miR-93 as the most suitable reference miR in both cohorts, either when analysed separately or collectively. Interestingly, there were large variat...Continue Reading

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Citations

Mar 22, 2016·Nature Reviews. Genetics·Sara A ByronDavid W Craig
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·G OcchipintiF Piva
Oct 30, 2016·Cellular Microbiology·Jin Kyung KimEun-Kyeong Jo
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Sep 1, 2019·Tuberculosis·Jessica L PedersenBernadette M Saunders
Feb 10, 2021·International Journal of Environmental Research and Public Health·Agnė ŠatrauskienėPaul Holvoet
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Jun 9, 2021·The Indian Journal of Tuberculosis·Priyanka Mehta
Aug 3, 2021·Frontiers in Molecular Biosciences·Gilles ParzibutJulien Guiot

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Methods Mentioned

BETA
PCR

Software Mentioned

GenEx
Normfinder
SDS Relative Quantification
geNorm

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