Identification of molecular signatures specific for distinct cranial sensory ganglia in the developing chick

Neural Development
Cedric PattheyJo Begbie

Abstract

The cranial sensory ganglia represent populations of neurons with distinct functions, or sensory modalities. The production of individual ganglia from distinct neurogenic placodes with different developmental pathways provides a powerful model to investigate the acquisition of specific sensory modalities. To date there is a limited range of gene markers available to examine the molecular pathways underlying this process. Transcriptional profiles were generated for populations of differentiated neurons purified from distinct cranial sensory ganglia using microdissection in embryonic chicken followed by FAC-sorting and RNAseq. Whole transcriptome analysis confirmed the division into somato- versus viscerosensory neurons, with additional evidence for subdivision of the somatic class into general and special somatosensory neurons. Cross-comparison of distinct ganglia transcriptomes identified a total of 134 markers, 113 of which are novel, which can be used to distinguish trigeminal, vestibulo-acoustic and epibranchial neuronal populations. In situ hybridisation analysis provided validation for 20/26 tested markers, and showed related expression in the target region of the hindbrain in many cases. One hundred thirty-four high-confi...Continue Reading

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Datasets Mentioned

BETA
SRP068496
HQ873432.1
EF552813.1
NR046276.1
DQ018753.1

Methods Mentioned

BETA
dissection
FACS
dissections
RNA-seq
fluorescence-activated cell sorting
PCA
Hi-seq
HTseq
PCR

Software Mentioned

MEGA
DESeq
Ensembl
BLASTp
GENBANK
DAVID
EdgeR
MAFFT
Gsnap
MISO

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