PMID: 6406225May 16, 1983Paper

Identification of monkey lung procathepsin D-II as a pepsinogen-C-like acid protease zymogen

European Journal of Biochemistry
A MoriyamaK Takahashi

Abstract

Procathepsin D-II (Mr = 37 500) was purified from Japanese monkey lung at pH 7.0, and was shown to be converted to the active form, cathepsin D-II (Mr = 33 000) via an intermediate (Mr = 35 500) upon treatment at pH 3.0 and 14 degrees C. Procathepsin D-II was shown to be the inactive precursor of cathepsin D-II based on the following results: the former was inactive toward heat-denaturated casein at pH 5.4 whereas the latter was active; the former was not inactivated by diazoacetyl-DL-norleucine methyl ester in the presence of Cu2+ ion at pH 6.0 whereas the latter was inactivated rapidly under the same conditions; and the former had no affinity to pepstatin-Sepharose between pH 5 and 7 whereas the latter was adsorbed to it. With a rabbit antiserum against procathepsin D-II, cathepsin D-II, pepsinogen C and pepsin C of Japanese monkey were each found to give a single precipitin line which fused completely with each other on agarose plate. On the other hand, cathepsin D-I purified from the monkey lung, and pepsinogens A (I, II, III-1, III-2 and III-3) obtained from the monkey gastric mucosa failed to precipitate with the antiserum. With the antiserum against the monkey pepsinogen C, the same results were obtained. Further, procat...Continue Reading

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Citations

Nov 16, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·K MatsusakoR Tanaka
Jun 14, 2013·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Kenji Takahashi
Sep 13, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Masao TanjiKenji Takahashi
Sep 1, 2006·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Yuichi NaritaTakashi Kageyama
Oct 1, 1987·Gut·P B SzecsiB Foltmann
Oct 28, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Cherie FosterSusan Guttentag
Jun 9, 2016·The Clinical Respiratory Journal·J Kyle BohmanDaryl J Kor
May 1, 1985·Analytical Biochemistry·B FoltmannN I Tarasova

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