Identification of monoclonal anti-HMW-MAA antibody linear peptide epitope by proteomic database mining

The Journal of Investigative Dermatology
Abraham MittelmanDarja Kanduc

Abstract

An efficient strategy is presented for the identification of antigenic sequences in the context of given MHC molecules of interest. The proteomic analysis of the antigenic peptide repertoire is described and demonstrated by using high-molecular weight melanoma-associated antigen. The identification of the epitopic sequence of a monoclonal antibody raised against the 250 kDa tumor associated antigen was reached by using only seven short synthetic peptide fragments, instead of the 155 non-overlapping 15-mer peptides theoretically necessary as minimum screening library. The present result has been obtained by applying as driving criteria the analysis of the peptide affinity to MHC class II molecules and the non-self discrimination concept.

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Citations

Dec 18, 2013·Immunotherapy·Jean Pierre Spinosa, Darja Kanduc
Jul 14, 2010·Journal of Biomedicine & Biotechnology·Guglielmo LuccheseDarja Kanduc
Dec 27, 2008·Experimental and Molecular Pathology·Angela Stufano, Darja Kanduc
Apr 7, 2007·Autoimmunity Reviews·Darja KanducAbraham Mittelman
Jun 11, 2005·Experimental Dermatology·Jörg WillersDarja Kanduc
Jun 15, 2012·Journal of Peptide Science : an Official Publication of the European Peptide Society·Darja Kanduc
Mar 21, 2012·Immunopharmacology and Immunotoxicology·G Lucchese, A Pesce Delfino
Oct 26, 2006·Journal of Translational Medicine·Giovanni AngeliniDarja Kanduc
May 26, 2018·Current Drug Discovery Technologies·Darja Kanduc
Nov 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alberta LuccheseReinhard Dummer
Oct 14, 2009·International Journal of Immunopathology and Pharmacology·A LuccheseD Kanduc
Aug 24, 2006·Journal of Translational Medicine·Alberta LuccheseDarja Kanduc

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