Identification of mosaicism in Prader-Willi syndrome using fluorescent in situ hybridization

American Journal of Medical Genetics
P A Mowery-RushtonU Surti

Abstract

We report on our findings of 4 patients with mosaicism for a deletion of chromosome 15, most commonly associated with Prader-Willi syndrome (PWS). We examined a series of typical and atypical PWS patients in order to identify cytogenetically undetected deletions, using fluorescence in situ hybridization. In 4 of the patients analyzed we detected a deletion in 14-60% of peripheral blood leukocytes, using four commercially available probes. Our results indicate that mosaicism may play a role in the etiology of some PWS cases. These findings may be especially useful in patients who display discrepancies between clinical phenotype and established diagnostic criteria. Methylation and microsatellite polymorphism analyses of 2 patients with low-level mosaicism failed to identify the deletion. We propose that fluorescence in situ hybridization is the most effective method for detecting somatic mosaicism, since a large number of cells can be individually examined for the presence or absence of a specific deletion.

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Citations

May 15, 1998·Journal of Medical Genetics·W P RobinsonA A Schinzel
May 13, 2010·BMC Medical Genetics·Simon C RamsdenKarin Buiting
Dec 23, 1999·American Journal of Medical Genetics·R D Nicholls
Jul 9, 1999·American Journal of Medical Genetics·W L GoldenT E Kelly
Jun 27, 2019·European Journal of Human Genetics : EJHG·Jasmin BeygoDeniz Kanber

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