Identification of mosquito bloodmeals using mitochondrial cytochrome oxidase subunit I and cytochrome b gene sequences

Medical and Veterinary Entomology
J S TownzenD D Judd

Abstract

Primer pairs were designed and protocols developed to selectively amplify segments of vertebrate mitochondrial cytochrome oxidase subunit 1 (COI) and cytochrome b (Cyt b) mtDNA from the bloodmeals of mosquitoes (Diptera: Culicidae). The protocols use two pairs of nested COI primers and one pair of Cyt b primers to amplify short segments of DNA. Resultant sequences are then compared with sequences in GenBank, using the BLAST function, for putative host identification. Vertebrate DNA was amplified from 88% of our sample of 162 wild-caught, blood-fed mosquitoes from Oregon, U.S.A. and GenBank BLAST searches putatively identified 98% of the amplified sequences, including one amphibian, seven mammalian and 14 avian species. Criteria and caveats for putative identification of bloodmeals are discussed.

References

Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T D KocherA C Wilson
Mar 1, 1988·Journal of Medical Entomology·W S Irby, C S Apperson
Nov 1, 1981·The American Journal of Tropical Medicine and Hygiene·T R BurkotG R DeFoliart
Jan 1, 1983·Annual Review of Entomology·R K Washino, C H Tempelis
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 8, 1999·Medical and Veterinary Entomology·D A BoakyeT R Unnasch
Dec 11, 1999·Nucleic Acids Research·D L WheelerB A Rapp
May 12, 2001·American Journal of Human Genetics·S FinniläK Majamaa
Aug 31, 2002·The American Journal of Tropical Medicine and Hygiene·Joon Hak LeeThomas R Unnasch
Mar 5, 2003·Proceedings. Biological Sciences·Paul D N HebertJeremy R deWaard
Apr 16, 2003·Journal of Medical Entomology·Kiet A Ngo, Laura D Kramer
Jan 1, 1957·British Journal of Preventive & Social Medicine·A W ANDERSONH J TRENCHARD
Mar 17, 2004·Vector Borne and Zoonotic Diseases·Charles S AppersonThomas R Unnasch
May 4, 2004·Molecular Phylogenetics and Evolution·Christian J LudtRalph Kuehn
Jul 16, 2004·Proceedings. Biological Sciences·Björn MalmqvistStaffan Bensch
Sep 30, 2004·PLoS Biology·Paul D N HebertCharles M Francis
Oct 11, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·K F Armstrong, S L Ball
Oct 26, 2005·Systematic Biology·Kipling W WillQuentin D Wheeler
Dec 31, 2005·Nucleic Acids Research·Dennis A BensonDavid L Wheeler
Jan 24, 2006·The Journal of Pain : Official Journal of the American Pain Society·Marshall Devor
May 18, 2006·Emerging Infectious Diseases·Goudarz MolaeiCharles R Vossbrinck
Jul 29, 2006·European Journal of Cardiovascular Prevention and Rehabilitation : Official Journal of the European Society of Cardiology, Working Groups on Epidemiology & Prevention and Cardiac Rehabilitation and Exercise Physiology·Tom Marshall
Jan 5, 2007·Nucleic Acids Research·Dennis A BensonDavid L Wheeler

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Citations

Oct 23, 2010·Parasitology Research·Camille NinioJerome Depaquit
Oct 11, 2013·Vector Borne and Zoonotic Diseases·Joseph O Mehus, Jefferson A Vaughan
Jan 19, 2012·PloS One·Kristine M SmithNina Marano
Dec 22, 2015·Medical and Veterinary Entomology·A C SchönenbergerC Silaghi
Oct 17, 2015·Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis·Hafiz Muhammad Tahir, Samreen Akhtar
Jan 15, 2013·Molecular Ecology Resources·Tae Jin JeongMyounghai Kwak
Aug 14, 2012·Medical and Veterinary Entomology·E VidevallR Ignell
Apr 10, 2016·Malaria Journal·Boubacar CoulibalyMichelle M Riehle
Dec 15, 2015·Frontiers in Veterinary Science·Leila Hadj-HenniDenis Augot
Apr 28, 2012·Current Biology : CB·Ida Bærholm SchnellM Thomas P Gilbert
Dec 3, 2014·Trends in Parasitology·Danielle A OndrejickaRobert H Hanner
Apr 27, 2016·Journal of Medical Entomology·Emily J FliesCraig R Williams
Nov 26, 2015·PloS One·Charles C Y XuCameron R Turner
Mar 30, 2017·ELife·Paul-Yannick Bitome-EssonoChristophe Paupy
Apr 18, 2018·PLoS Neglected Tropical Diseases·Meriem LouniOleg Mediannikov
Oct 3, 2017·Journal of Medical Entomology·Lee P McPhatterAlec C Gerry
Dec 12, 2018·The American Journal of Tropical Medicine and Hygiene·Adama Zan DiarraPhilippe Parola
Mar 15, 2019·Emerging Microbes & Infections·Ahmed KandeilGhazi Kayali
Jun 27, 2019·Archives of Virology·Joseph DiffoChristian E Lange
Apr 10, 2020·PloS One·Marc T ValituttoJonna Mazet
Aug 29, 2018·Nature Microbiology·Tracey GoldsteinJonna A K Mazet
Feb 1, 2013·The Journal of General Virology·S J AnthonyW I Lipkin
Dec 8, 2019·EcoHealth·Julius NzizaKirsten Gilardi

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