PMID: 9437708Jan 1, 1997Paper

Identification of motilin mRNA in the brain of man and rabbit. Conservation of polymorphism of the motilin gene across species

Peptides
Inge DepoortereTheo L Peeters

Abstract

The data regarding the identity of motilin-like immunoreactivity in the central nervous system are controversial. The aim of the present study was to clarify whether motilin mRNA is present in the brain of rabbit and man. Total RNA, prepared from several regions of the rabbit brain, was subjected to RT-PCR aimed at amplifying a 294 bp cDNA fragment of the rabbit motilin precursor. The amplified product was subcloned and sequenced. The sequence showed 7 differences compared to the one reported for the duodenal precursor (1). However the duodenal precursor from the rabbit used in the present study revealed identical substitutions. One of these, involving amino acid -11 of the signal peptide, was shown to be due to gene polymorphism, as has also been described at this site in man. By radioimmunoassay the highest concentration of motilin (fmol/mg protein) was detected in the hippocampus (4788 +/- 295), the lowest in the telencephalon (2127 +/- 221). Using a similar approach, but starting from commercial human brain mRNA, the sequence of a comparable cDNA fragment of the human brain motilin precursor was obtained. Its sequence was identical with the one published for the human intestinal precursor (41). Our study demonstrates that m...Continue Reading

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Citations

Aug 23, 2003·Brain Research·Yanfang GuanTheo L Peeters
Jul 1, 2009·Journal of Clinical Gastroenterology·Noriyuki HataHiroyuki Okano
Nov 17, 2009·Annales Françaises D'anesthèsie Et De Rèanimation·N LibertL Brinquin
Nov 12, 2015·Biological Psychiatry·Scott E Kanoski, Harvey J Grill
Aug 25, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·H LiuY Chen
May 12, 2009·Pharmacology & Therapeutics·Betty De SmetInge Depoortere
Jan 29, 2016·The American Journal of Clinical Nutrition·Eveline DelooseJan Tack
Aug 3, 2001·Nature Reviews. Neuroscience·A Inui
Jan 31, 2020·Expert Review of Gastroenterology & Hepatology·Kanageswari SingaramMaxim S Petrov
May 12, 2021·Ocular Immunology and Inflammation·Richard R SadigKenneth G-J Ooi

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