PMID: 6170984Sep 1, 1981Paper

Identification of mouse mammary epithelial cells by immunofluorescence with rabbit and guinea pig antikeratin antisera

Proceedings of the National Academy of Sciences of the United States of America
B B AschT T Sun

Abstract

Few markers are available to identify the three types of mammary epithelial cells--ductal, alveolar, and myoepithelial--especially in pathological conditions and in cell cultures. We have used antisera to human keratins in immunofluorescence to facilitate the identification of the three mouse mammary epithelial cell types. In frozen tissue sections and primary cell cultures, a rabbit antikeratin antiserum specifically stained cytoplasmic filaments in all three types of epithelial cells. A guinea pig antiserum against the same keratin preparation, however, reacted preferentially with filaments in myoepithelial cells and readily detected this cell type in normal, dysplastic, and malignant mammary tissues and cell cultures. Neither antisera reacted with fibroblasts or any other mesenchymal cells. The combined use of the two antikeratin antisera thereby permits rapid surveys of tissue sections and cultures for the localization of not only all epithelial cells but also the subpopulation of myoepithelial cells. Moreover, when mammary cultures established from late-pregnant or lactating mice were stained simultaneously with guinea pig antikeratin and rabbit anticasein antisera, three populations of epithelial cells were mutually exclu...Continue Reading

References

Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T T SunH Green
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J M Starger, R D Goldman
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M OsbornK Weber
Jan 1, 1978·Cell·R O Hynes, A T Destree
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W W FrankeK Weber
Dec 11, 1979·Biochemistry·P M Steinert, W W Idler
Mar 1, 1980·The Journal of Cell Biology·W W FrankeT W Keenan
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P BannaschW W Franke

❮ Previous
Next ❯

Citations

Jan 1, 1989·Virchows Archiv. A, Pathological Anatomy and Histopathology·A TsuburaS H Dairkee
Nov 1, 1989·The Histochemical Journal·M J WarburtonP S Rudland
Aug 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J M Raber, S M D'Ambrosio
Jan 1, 1984·Biochimica Et Biophysica Acta·H S SmithA J Hackett
Dec 1, 1984·The Journal of Laryngology and Otology·S R ShiM L Goodman
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·W W FrankeH Denk
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K G DanielsonD Medina
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R AllenB Armstrong
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S H DairkeeA J Hackett
Mar 1, 1984·The Journal of Cell Biology·U K EhmannD S Misfeldt
Sep 1, 1990·Zentralblatt für Veterinärmedizin. Reihe A·L PeñaM Gonzalez
Jul 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·N W ShappellB B Asch
May 1, 1989·Zentralblatt für Veterinärmedizin. Reihe A·J H VosM de Neijs
Apr 1, 1990·Differentiation; Research in Biological Diversity·C MørkO W Petersen
Sep 1, 1988·Journal of Oral Pathology·U KjörellL E Thornell
Sep 1, 1987·Journal of Dairy Science·M F McGrath
Nov 1, 1985·Journal of Cellular Physiology·M McGrathS Nandi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.