Identification of mouse orthologue of endogenous secretory receptor for advanced glycation end-products: structure, function and expression

The Biochemical Journal
Ai HarashimaH Yamamoto

Abstract

The cell-surface RAGE [receptor for AGE (advanced glycation end-products)] is associated with the development of diabetic vascular complications, neurodegenerative disorders and inflammation. Recently, we isolated a human RAGE splice variant, which can work as a decoy receptor for RAGE ligands, and named it esRAGE (endogenous secretory RAGE). In the present study, we have isolated the murine equivalent of esRAGE from brain polysomal poly(A)+ (polyadenylated) RNA by RT (reverse transcription)-PCR cloning. The mRNA was generated by alternative splicing, and it encoded a 334-amino-acid protein with a signal sequence, but lacking the transmembrane domain. A transfection experiment revealed that the mRNA was actually translated as deduced to yield the secretory protein working as a decoy in AGE-induced NF-kappaB (nuclear factor kappaB) activation. RT-PCR and immunoblotting detected esRAGE mRNA and protein in the brain, lung, kidney and small intestine of wild-type mice, but not of RAGE-null mice. The esRAGE expression was increased in the kidney of diabetic wild-type mice. The present study has thus provided an animal orthologue of esRAGE for clarification of its roles in health and disease.

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Citations

Nov 26, 2013·Genome Biology and Evolution·Raquel López-DíezBegoña Aguado
Jan 24, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anastasia Z KaleaBarry I Hudson
Mar 28, 2012·Journal of Diabetes Investigation·Yasuhiko Yamamoto, Hiroshi Yamamoto
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Jun 7, 2008·American Journal of Respiratory and Critical Care Medicine·Haiying ZhangAkitoshi Ishizaka
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Jul 22, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Lin LuWei Feng Shen
Aug 24, 2007·Biochimica Et Biophysica Acta·Mari OsawaHiroshi Yamamoto
Oct 16, 2014·Proteomics·Kedar B BatkulwarMahesh J Kulkarni
Jul 29, 2011·Journal of Zhejiang University. Science. B·Lin LuWei-Feng Shen
Aug 5, 2008·Respiratory Physiology & Neurobiology·Tapan K MukherjeeJohn R Hoidal
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Feb 22, 2011·Journal of the American Society of Nephrology : JASN·Diane L Rosin, Mark D Okusa
Nov 7, 2019·International Journal of Molecular Sciences·Juria AkasakaHiroshi Kobayashi
Jul 8, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Angela RaucciMarco E Bianchi
Feb 6, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hanbyeol LeeSe-Ran Yang
Jan 29, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yasuhiko YamamotoHiroshi Yamamoto
Jul 20, 2017·European Journal of Clinical Investigation·Ting-Wei LeeYi-Jen Chen
Sep 19, 2019·Biochimica Et Biophysica Acta. General Subjects·Babett BartlingAndreas Simm
Apr 28, 2007·Diabetes Research and Clinical Practice·Yasuhiko YamamotoHiroshi Yamamoto

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