Identification of mouse serum miRNA endogenous references by global gene expression profiles.

PloS One
Qing-Sheng MiLi Zhou

Abstract

MicroRNAs (miRNAs) are recently discovered small non-coding RNAs and can serve as serum biomarkers for disease diagnosis and prognoses. Lack of reliable serum miRNA endogenous references for normalization in miRNA gene expression makes single miRNA assays inaccurate. Using TaqMan® real-time PCR miRNA arrays with a global gene expression normalization strategy, we have analyzed serum miRNA expression profiles of 20 female mice of NOD/ShiLtJ (n = 8), NOR/LtJ (n = 6), and C57BL/6J (n = 6) at different ages and disease conditions. We identified five miRNAs, miR-146a, miR-16, miR-195, miR-30e and miR-744, to be stably expressed in all strains, which could serve as mouse serum miRNA endogenous references for single assay experiments.

References

Dec 1, 1975·Journal of Immunological Methods·S A LeonB Shapiro
Dec 19, 2003·Nucleic Acids Research·Sam Griffiths-Jones
Nov 16, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Jacques B de KokPaul N Span
Mar 2, 2005·Seminars in Immunology·Chang-Zheng Chen, Harvey F Lodish
Aug 30, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yu-Kwan Tong, Y M Dennis Lo
Dec 31, 2005·Nucleic Acids Research·Sam Griffiths-JonesAnton J Enright
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Oct 25, 2006·The Journal of Experimental Medicine·Bradley S CobbMatthias Merkenschlager
Oct 25, 2006·Nature Reviews. Cancer·George A Calin, Carlo M Croce
Jun 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·Scott ShellMarcus E Peter
Nov 10, 2007·Nucleic Acids Research·Sam Griffiths-JonesAnton J Enright
Jul 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrick S MitchellMuneesh Tewari
Sep 6, 2008·PloS One·Shlomit GiladAyelet Chajut
Feb 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kai WangDavid J Galas
Jun 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Li ZhouQing-Sheng Mi
Jun 23, 2009·International Immunopharmacology·Li ZhouQing-Sheng Mi
Aug 22, 2009·Clinical Chemistry·Xu JiNaoharu Iwai
Sep 12, 2009·Clinical Chemistry·Omar F LaterzaWarren E Glaab
Oct 31, 2009·International Journal of Cancer. Journal International Du Cancer·Zhaohui HuangXiang Du
Feb 12, 2010·Methods : a Companion to Methods in Enzymology·Evan M KrohMuneesh Tewari
Mar 18, 2010·British Journal of Cancer·M TsujiuraE Otsuji
Jun 11, 2010·European Heart Journal·Yuri D'AlessandraMaurizio C Capogrossi
Jul 20, 2010·Biochimica Et Biophysica Acta·Jürgen Wittmann, Hans-Martin Jäck
Nov 3, 2010·Nucleic Acids Research·Ana Kozomara, Sam Griffiths-Jones
Mar 15, 2014·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Masahiro TsujiuraEigo Otsuji

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Citations

Sep 21, 2013·Experimental & Molecular Medicine·Fabian BenzTom Luedde
Jan 10, 2014·Experimental Dermatology·Yu-Ling ShiLi Zhou
May 2, 2015·Biosensors & Bioelectronics·Yanting ShenZuhong Lu
Jul 28, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Alison H HarrillBrian N Chorley
Oct 27, 2016·Experimental Dermatology·Qing LiuQing-Sheng Mi
Feb 6, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Parvathi RanganathanRamiro Garzon
Oct 24, 2019·Hepatology International·Shuang RenPing Liu
May 30, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maayke G M KokSara-Joan Pinto-Sietsma
Jun 5, 2020·JCI Insight·Paulina PodkalickaJózef Dulak
Sep 19, 2019·American Journal of Physiology. Endocrinology and Metabolism·Shuyue WangYuxin Li
Mar 7, 2020·Scientific Reports·Giovanni Luca RomanoClaudio Bucolo

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Methods Mentioned

BETA
PCR

Software Mentioned

RQ manager
Statminer®
SDS
SPSS

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