Abstract
The mouse zona pellucida (ZP) protein ZP3 plays an important role in the process of fertilization by mediating sperm binding and the acrosome reaction. ZP3 primary structures are highly conserved, as revealed by cDNA cloning. We raised antisera against synthetic peptides that are either conserved in the structure of ZP3 from different mammalian species (AS ZP3-5 and AS ZP3-6) or specific for mouse ZP3 (AS ZP3-2). In ovary sections, AS ZP3-2 revealed immunoreactivity only to mouse ZP. AS ZP3-5 and AS ZP3-6 reacted with mouse, human, rat, hamster, porcine, and bovine ZP proteins. In porcine oocytes, immunoreactive material was highly abundant in the ooplasm. Immunoblots showed that antiserum AS ZP3-5 recognized the mouse ZP3 protein. In porcine ZP preparations, AS ZP3-5 recognized a 53-kDa ZP protein. No reaction was observed with purified porcine ZP3 alpha or with ZP3 beta. Immunofluorescence studies revealed that AS ZP3-5 and AS ZP3-6 antibodies react with ZP of isolated porcine and human oocytes. Our results show that antisera against synthetic mouse ZP3 peptides can be used as markers for the identification of ZP3-like proteins in mammalian oocytes and might be useful tools for the evaluation of ZP integrity and ZP3 function.
Citations
Sep 27, 2003·Theriogenology·Elvira HinschKlaus Dieter Hinsch
Dec 4, 2001·Journal of Reproductive Immunology·Scott A CoonrodJohn C Herr
Jun 1, 1997·Journal of Reproductive Immunology·A AfzalpurkarS K Gupta
Apr 1, 1999·Human Reproduction·E HinschK D Hinsch
Jan 29, 2009·Human Reproduction·N DesaiT Falcone
Feb 28, 2003·Biology of Reproduction·Ruo-Lan XiangJing-Pian Peng
Oct 1, 1994·American Journal of Reproductive Immunology : AJRI·K D HinschS Oehninger
Dec 14, 2011·Clinical & Developmental Immunology·Wan-Xiang XuYi Xie
Oct 30, 2012·Ontogenez·I N RozhkovaS Ia Amstislavskiĭ
Nov 16, 2010·Theriogenology·M J Izquierdo-RicoM Avilés
Nov 19, 2010·Journal of Biotechnology·Ya-ping HeYi Xie
Mar 15, 2005·Theriogenology·E HinschK-D Hinsch
Jul 31, 2012·Journal of Proteomics·I StetsonM Avilés
Apr 17, 2001·American Journal of Obstetrics and Gynecology·K W DongS Oehninger
Feb 9, 2002·Molecular Reproduction and Development·Chang Hun KimHideaki Tojo
Jul 10, 2009·Biology of Reproduction·Sylvie MugnierGhylène Goudet
Jul 1, 2014·Experimental Animals·Masaru Okabe
Sep 18, 1998·Andrologia·D R Franken
Sep 18, 1998·Andrologia·E HinschK D Hinsch
Aug 2, 2018·PeerJ·Yeyu ChenXiaochuan Deng
Apr 20, 2001·Biochimica Et Biophysica Acta·K D HinschL Konrad
Jan 30, 2004·Reproductive Biomedicine Online·Sergio Oehninger