Identification of mu- and kappa-opioid receptors as potential targets to regulate parasympathetic, sympathetic, and sensory neurons within rat intracardiac ganglia

The Journal of Comparative Neurology
Shaaban A MousaMichael Schäfer

Abstract

Recent interest has been focused on the opioid regulation of heart performance; however, specific allocation of opioid receptors to the parasympathetic, sympathetic, and sensory innervations of the heart is scarce. Therefore, the present study aimed to characterize such specific target sites for opioids in intracardiac ganglia, which act as a complex network for the integration of the heart's neuronal in- and output. Tissue samples from rat heart atria were subjected to RT-PCR, Western blot, radioligand-binding, and double immunofluorescence confocal analysis of mu (M)- and kappa (K)-opioid receptors (ORs) with the neuronal markers vesicular acetylcholine transporter (VAChT), tyrosine hydroxylase (TH), calcitonin gene-related peptide (CGRP), and substance P (SP). Our results demonstrated MOR- and KOR-specific mRNA, receptor protein, and selective membrane ligand binding. By using immunofluorescence confocal microscopy, MOR and KOR immunoreactivity were colocalized with VAChT in large-diameter parasympathetic principal neurons, with TH-immunoreactive small intensely fluorescent (SIF) cells, and on nearby TH-IR varicose terminals. In addition, MOR and KOR immunoreactivity were identified on CGRP- and SP-IR sensory neurons through...Continue Reading

References

Aug 1, 1987·Journal of the Autonomic Nervous System·B J PardiniD D Lund
Jun 25, 1995·Journal of the Autonomic Nervous System·J C HardwickR L Parsons
Jan 26, 1996·Biochemical and Biophysical Research Communications·G WittertD Pyle
Oct 1, 1996·The Journal of Thoracic and Cardiovascular Surgery·S SinghR D Wurster
Aug 1, 1997·Brain Research·R E CoggeshallS M Carlton
Jun 30, 2000·Clinical and Experimental Pharmacology & Physiology·M J ChristieE E Bagley
Dec 29, 2000·Journal of the American College of Cardiology·S P BellD M Yellon
Apr 24, 2001·Pharmacology & Therapeutics·J E Schultz, G J Gross
Mar 1, 2002·Experimental Neurology·Pablo R BrumovskyTomas Hökfelt
Jan 11, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Martin FariasJames L Caffrey
Jun 24, 2003·Journal of Molecular and Cellular Cardiology·Garrett J Gross
Jul 26, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Martin FariasJames L Caffrey
Nov 1, 2003·The Journal of Pharmacology and Experimental Therapeutics·Mohammed A ShaquraMichael Schäfer
Apr 28, 2005·Current Pharmaceutical Design·U Barry, Z Zuo
Dec 5, 2006·Brain : a Journal of Neurology·Shaaban A MousaMichael Schäfer
Mar 21, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Ming-He HuangBarry F Uretsky
Oct 24, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Eric W DicksonGerene M Denning
Nov 23, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Shekhar H DeoJames L Caffrey
Feb 5, 2008·The Journal of Clinical Investigation·Christian ZöllnerMichael Schäfer
Jun 3, 2008·Archives of Medical Research·Liu Jin-ChengPei Jian-Ming

❮ Previous
Next ❯

Citations

Jan 7, 2014·Heart and Vessels·Piotr SobanskiShaaban A Mousa
May 19, 2011·Biochemistry. Biokhimii︠a︡·Yu A Kovalitskaya, E V Navolotskaya
May 21, 2016·Medicinal Research Reviews·Leonid N MaslovJames M Downey
Apr 30, 2013·The Journal of Pain : Official Journal of the American Pain Society·Mohammed ShaquraMichael Schäfer
Jan 25, 2017·International Journal of Molecular Sciences·Mary MallappallilMoro Salifu
Jan 24, 2021·Cardiovascular Drugs and Therapy·Lukas DeheSascha Treskatsch

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.