Identification of multiple actin-binding sites in cofilin-phosphatase Slingshot-1L

FEBS Letters
Masahiro YamamotoKensaku Mizuno

Abstract

Slingshot-1L (SSH1L) is a phosphatase that specifically dephosphorylates and activates cofilin, an actin-severing and -depolymerizing protein. SSH1L binds to and is activated by F-actin in vitro, and co-localizes with F-actin in cultured cells. We examined the F-actin-binding activity, F-actin-mediated phosphatase activation, and subcellular distribution of various mutants of SSH1L. We identified three sites involved in F-actin binding of SSH1L: Trp-458 close to the C-terminus of the phosphatase domain, an LHK motif in the N-terminal region, and an LKR motif in the C-terminal region. These sites play unique roles in the control of subcellular localization and F-actin-mediated activation of SSH1L.

References

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Feb 26, 2003·Cell·Thomas D Pollard, Gary G Borisy
May 26, 2004·The Journal of Cell Biology·Kyoko Nagata-OhashiKensaku Mizuno

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Citations

Jan 12, 2012·The Journal of Biological Chemistry·Jack W HowarthPaul R Rosevear
Aug 27, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Rebecca A TorresDavid S Weber
Jan 25, 2012·The American Journal of the Medical Sciences·Jian-Wu ZhaoYan Wang
Jul 25, 2009·Free Radical Biology & Medicine·David I Brown, Kathy K Griendling
Dec 17, 2008·Journal of Molecular Biology·Henri S SalehHelen Morrison
Oct 16, 2007·Seminars in Cancer Biology·Mario Gimona
May 31, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Souichi KuritaKensaku Mizuno
Apr 29, 2011·EMBO Reports·Sandra BarišićAngelika Hausser
Dec 3, 2014·Biochemical and Biophysical Research Communications·Katsunori TakahashiKensaku Mizuno
Jan 30, 2015·Nucleic Acids Research·Ana Lilia Torres-MachorroLorraine Pillus
Apr 7, 2010·The Journal of Biological Chemistry·Tim EiselerKlaus Pfizenmaier
May 17, 2007·Journal of Cell Science·Eleonora JovcevaJohn F Timms
May 24, 2008·European Journal of Cell Biology·Marleen Van TroysChristophe Ampe

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