Abstract
Slingshot-1L (SSH1L) is a phosphatase that specifically dephosphorylates and activates cofilin, an actin-severing and -depolymerizing protein. SSH1L binds to and is activated by F-actin in vitro, and co-localizes with F-actin in cultured cells. We examined the F-actin-binding activity, F-actin-mediated phosphatase activation, and subcellular distribution of various mutants of SSH1L. We identified three sites involved in F-actin binding of SSH1L: Trp-458 close to the C-terminus of the phosphatase domain, an LHK motif in the N-terminal region, and an LKR motif in the C-terminal region. These sites play unique roles in the control of subcellular localization and F-actin-mediated activation of SSH1L.
References
Jul 10, 1992·Cell·E FriederichD Louvard
Feb 7, 1998·The Journal of Cell Biology·G VaduvaA K Hopper
Jul 9, 1998·Nature·S ArberP Caroni
Jul 9, 1998·Nature·N YangK Mizuno
Aug 26, 1999·Trends in Cell Biology·J R BamburgS Ono
Apr 11, 2001·Molecular Biology of the Cell·J ToshimaK Mizuno
Feb 8, 2002·Cell·Ryusuke NiwaTadashi Uemura
Feb 26, 2003·Cell·Thomas D Pollard, Gary G Borisy
Oct 9, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yusaku OhtaKensaku Mizuno
Dec 3, 2003·The Journal of Biological Chemistry·Michiru NishitaKensaku Mizuno
May 26, 2004·The Journal of Cell Biology·Kyoko Nagata-OhashiKensaku Mizuno
Jan 22, 2005·The EMBO Journal·Juliana SoosairajahOra Bernard
Oct 19, 2005·The Journal of Cell Biology·Michiru NishitaKensaku Mizuno
Citations
Mar 31, 2009·Nature Cell Biology·Tim EiselerPeter Storz
Jan 12, 2012·The Journal of Biological Chemistry·Jack W HowarthPaul R Rosevear
Sep 13, 2007·The Journal of Biological Chemistry·Kristina KligysJonathan C R Jones
May 28, 2013·Antioxidants & Redox Signaling·Florian VeitNorbert Weissmann
Aug 27, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Rebecca A TorresDavid S Weber
Jun 10, 2010·PLoS Biology·Maud HertzogGiorgio Scita
Jan 25, 2012·The American Journal of the Medical Sciences·Jian-Wu ZhaoYan Wang
Nov 17, 2012·Cellular Signalling·Kensaku Mizuno
Jul 25, 2009·Free Radical Biology & Medicine·David I Brown, Kathy K Griendling
Dec 17, 2008·Journal of Molecular Biology·Henri S SalehHelen Morrison
Oct 16, 2007·Seminars in Cancer Biology·Mario Gimona
May 31, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Souichi KuritaKensaku Mizuno
Apr 29, 2011·EMBO Reports·Sandra BarišićAngelika Hausser
Dec 3, 2014·Biochemical and Biophysical Research Communications·Katsunori TakahashiKensaku Mizuno
Feb 17, 2015·Cancer Letters·Yufeng WangKazuyuki Nakamura
Jan 30, 2015·Nucleic Acids Research·Ana Lilia Torres-MachorroLorraine Pillus
Jul 3, 2016·Andrology·S M ChenY X Ma
Apr 7, 2010·The Journal of Biological Chemistry·Tim EiselerKlaus Pfizenmaier
Oct 17, 2017·Oncotarget·Chen ChenHuang Tao
Sep 24, 2008·The Journal of Biological Chemistry·Souichi KuritaKensaku Mizuno
Sep 5, 2014·PLoS Pathogens·Harald BieligThomas A Kufer
Nov 21, 2014·Development·Takashi AbeTetsuya Tabata
May 17, 2007·Journal of Cell Science·Eleonora JovcevaJohn F Timms
May 24, 2008·European Journal of Cell Biology·Marleen Van TroysChristophe Ampe