Identification of multiple isomeric core chitobiose-modified high-mannose and paucimannose N -glycans in the planarian Schmidtea mediterranea

The Journal of Biological Chemistry
Sabarinath Peruvemba SubramanianRamaswamy Subramanian

Abstract

Cell surface-associated glycans mediate many cellular processes, including adhesion, migration, signaling, and extracellular matrix organization. The galactosylation of core fucose (GalFuc epitope) in paucimannose and complex-type N-glycans is characteristic of protostome organisms, including flatworms (planarians). Although uninvestigated, the structures of these glycans may play a role in planarian regeneration. Whole-organism MALDI-MS analysis of N-linked oligosaccharides from the planarian Schmidtea mediterranea revealed the presence of multiple isomeric high-mannose and paucimannose structures with unusual mono-, di-, and polygalactosylated (n = 3-5) core fucose structures; the latter structures have not been reported in other systems. Di- and trigalactosylated core fucoses were the most dominant glycomers. N-Glycans showed extensive, yet selective, methylation patterns, ranging from non-methylated to polymethylated glycoforms. Although the majority of glycoforms were polymethylated, a small fraction also consisted of non-methylated glycans. Remarkably, monogalactosylated core fucose remained unmethylated, whereas its polygalactosylated forms were methylated, indicating structurally selective methylation. Using database se...Continue Reading

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Citations

Aug 15, 2019·Biological Reviews of the Cambridge Philosophical Society·Harry C TjondroMorten Thaysen-Andersen
May 3, 2019·Parasitology·Katharina Paschinger, Iain B H Wilson
Sep 10, 2021·Glycobiology·Sabarinath Peruvemba SubramanianRamaswamy Subramanian
Dec 8, 2021·Biological Reviews of the Cambridge Philosophical Society·Charlotte ToustouMuriel Bardor

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Methods Mentioned

BETA
single cell sequencing
NMR
PCR

Software Mentioned

SMART
MEGA7
Data Explorer
BLASTp
cellSens Dimension
ESPript
MUSCLE
GlycoWorkbench

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