Identification of multiple nuclear localization signals in murine Elf3, an ETS transcription factor

FEBS Letters
Hyun-Jin DoJae-Hwan Kim

Abstract

We investigated nuclear localization signal (NLS) determinants within the AT-hook and ETS DNA-binding domains of murine Elf3 (mElf3), a member of the subfamily of epithelium-specific ETS transcription factors. Deletion mutants containing the AT-hook, ETS domain or both localized strictly in the nucleus, suggesting that these individual domains contain independent NLS motif(s). Within the AT-hook domain, four basic residues (244KRKR247) were critical for strong NLS activity, and two potent bipartite NLS motifs (236-252 and 249-267) were sufficient for nuclear import of mElf3, although less efficient than the full domain. In addition, one stretch of basic residues (318KKK320) within the ETS domain appears to be essential for mElf3 nuclear localization. Taken together, mElf3 contains multiple NLS motifs, which may function cooperatively to effect efficient nuclear transport.

References

Jan 17, 1986·Cell·W D RichardsonA E Smith
Oct 1, 1988·Molecular and Cellular Biology·C V Dang, W M Lee
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·J F Maher, D Nathans
Aug 26, 1998·Advances in Cancer Research·B J Graves, J M Petersen
Sep 22, 1998·Nucleic Acids Research·L Aravind, D Landsman
Dec 28, 1999·Annual Review of Cell and Developmental Biology·D Görlich, U Kutay
Jun 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D A JansM H Lam
Dec 11, 2002·Essays in Biochemistry·E D Schwoebel, M S Moore
Feb 1, 2003·Gene·Tsuneyuki Oikawa, Toshiyuki Yamada
Sep 19, 2003·Current Genetics·Rebekah Burich, Ming Lei
Jun 1, 2004·Molecular and Cellular Biology·Jason D PrescottArthur Gutierrez-Hartmann

❮ Previous
Next ❯

Citations

Oct 6, 2009·The Journal of Biological Chemistry·Min-Chul KwonYoung-Yun Kong
Feb 2, 2008·The Journal of Biological Chemistry·Melanie TheodoreIfeanyi J Arinze
Dec 27, 2007·Cell Structure and Function·Wakana Sugano, Masamitsu Yamaguchi
Jul 6, 2014·Journal of Biochemistry·Yanhua JinYeun Kyu Jang
Jun 6, 2009·The Journal of Biological Chemistry·Wenbo LinTao Tao
Sep 20, 2008·Analytical Biochemistry·Takeshi NamikiVincent J Hearing
Jan 2, 2007·Biochemical and Biophysical Research Communications·Hyun-Jin DoJae-Hwan Kim
Dec 23, 2006·Human Molecular Genetics·James E J BedardStephanie M Ware

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.