Identification of multiple post-translational modifications in the porcine brain specific p25alpha

Journal of Neurochemistry
Anne J KleinnijenhuisPoul Henning Jensen

Abstract

P25alpha is a protein normally expressed in oligodendrocytes and subcellular relocalization of p25alpha occurs in multiple system atrophy, Parkinson's disease and Lewy body dementia along with ectopic expression in neurons. Moreover, it accumulates in Lewy body inclusions with aggregated alpha-synuclein and is a potent stimulator of alpha-synuclein aggregation. P25alpha is a phosphoprotein and post-translational modifications (PTMs) may play a role in its disease-related abnormalities. To investigate the spectrum of PTMs on p25alpha we cloned porcine p25alpha and isolated the protein from porcine brain. Using several complementary tandem mass spectrometry techniques for peptide mass analysis and amino acid sequencing, a comprehensive analysis of the PTMs on porcine p25alpha was performed. It was found that porcine p25alpha is heavily modified with a variety of modifications: phosphorylation, di- and trimethylation, citrullination and a HexNAc group. The modifications are localized within p25alpha's unfolded terminal domains and suggest that their functional states are regulated. This comprehensive mapping of p25alpha's PTMs will form the basis for future functional studies and investigations of p25alpha's potential role as a bi...Continue Reading

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Citations

Feb 10, 2012·Proteomics·Marlies De CeuleneerDieter Deforce
Apr 22, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Judit Ovádi, Ferenc Orosz
Dec 27, 2019·Immunological Reviews·Justyna Fert-BoberFelipe Andrade
Dec 25, 2019·Analytical and Bioanalytical Chemistry·Anne J KleinnijenhuisYohann Wittrant
Sep 18, 2012·Journal of Proteome Research·Marlies De CeuleneerDieter Deforce

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