Identification of mumps virus protein and lipid composition by mass spectrometry

Virology Journal
Marija BrglesMartina Marchetti-Deschmann

Abstract

Mumps virus is a negative-sense, single stranded RNA virus consisting of a ribonucleocapsid core enveloped by a lipid membrane derived from host cell, which causes mumps disease preventable by vaccination. Since virus lipid envelope and glycosylation pattern are not encoded by the virus but dependent on the host cell at least to some extent, the aim of this work was to analyse L-Zagreb (L-Zg) mumps virus lipids and proteins derived from two cell types; Vero and chicken embryo fibroblasts (CEF). Jeryl Lynn 5 (JL5) mumps strain lipids were also analysed. Virus lipids were isolated by organic phase extraction and subjected to 2D-high performance thin layer chromatography followed by lipid extraction and identification by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Virus samples were also subjected to gel electrophoresis under denaturating conditions and protein bands were excised, in-gel trypsinized and identified by MS as well as tandem MS. Results showed that lipids of both mumps virus strains derived from Vero cells contained complex glycolipids with up to five monosaccharide units whereas the lipid pattern of mumps virus derived from CEF was less complex. Mumps virus was found to contain expected ...Continue Reading

References

Dec 1, 1978·The Journal of General Virology·C Orvell
Feb 1, 1976·Journal of Virology·S C Jensik, S Silver
Sep 1, 1990·Journal of Virology·R G Paterson, R A Lamb
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R C AloiaL M Gordon
Mar 1, 1971·Journal of Virology·H D Klenk, P W Choppin
Feb 1, 1980·The Journal of General Virology·B K RimaS J Martin
Jun 1, 1982·Virology·G Herrler, R W Compans
Jan 1, 1980·The Journal of General Virology·M McCarthy, R T Johnson
Jun 1, 1980·The Journal of General Virology·M McCarthy, R T Johnson
Feb 1, 1981·Virology·O P Zhirnov, A G Bukrinskaya
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R C AloiaF C Jensen
Nov 20, 2001·Chemical Reviews·R Aebersold, D R Goodlett
May 14, 2003·Journal of Virology·Christina M UlaneCurt M Horvath
Jul 30, 2003·The Journal of Cell Biology·Annegret Pelchen-MatthewsMark Marsh
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Jul 29, 2004·Journal of Virology·Richard L KingstonLeslie S Gay
Sep 29, 2004·Journal of Virology·Susan M VarnumJay A Nelson
Sep 29, 2004·Journal of Virology·Lisa M KattenhornBenedikt M Kessler
Nov 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Eric JohannsenElliott Kieff
Mar 16, 2005·Journal of Virology·Toru KubotaAtsushi Kato
Jan 28, 2006·Journal of Virology·Rebecca L WilsonBiao He
Feb 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Britta BrüggerHans-Georg Kräusslich
Aug 15, 2006·Biochemical and Biophysical Research Communications·Yueyong LiuPo Tien
Nov 14, 2006·Nucleic Acids Research·Manish SudShankar Subramaniam
Apr 3, 2007·Trends in Microbiology·David J Vigerust, Virginia L Shepherd
Apr 17, 2007·FEBS Letters·Sonja WelschHans-Georg Kräusslich
Jul 4, 2008·Journal of Virology·Sandra LoretRoger Lippé
Feb 4, 2010·Virology·Jinlu WuChoy-Leong Hew

❮ Previous
Next ❯

Citations

Aug 25, 2016·Molecular Therapy Oncolytics·Arun AmmayappanMark J Federspiel
Dec 27, 2019·Mass Spectrometry Reviews·Aleksandra MilewskaPiotr Suder
Oct 18, 2018·Virology Journal·Dora SvibenMarija Brgles
Apr 23, 2021·Journal of the American Society for Mass Spectrometry·Anh TranJace W Jones

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
electrophoresis
by density gradient
immunoprecipitation
acetylation
FCS

Software Mentioned

Flexanalysis
mMass
Biotools
UltrafleXtreme

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.