Identification of mutator genes and mutational pathways in Escherichia coli using a multicopy cloning approach

Molecular Microbiology
Hanjing YangJeffrey H Miller

Abstract

We searched for genes that create mutator phenotypes when put on to a multicopy plasmid in Escherichia coli. In many cases, this will result in overexpression of the gene in question. We constructed a random shotgun library with E. coli genomic fragments between 3 and 5 kbp in length on a multicopy plasmid vector that was transformed into E. coli to screen for frameshift mutators. We identified a total of 115 independent genomic fragments that covered 17 regions on the E. coli chromosome. Further studies identified 12 genes not previously known as causing mutator phenotypes when overproduced. A striking finding is that overproduction of the multidrug resistance transcription regulator, EmrR, results in a large increase in frameshift and base substitution mutagenesis. This suggests a link between multidrug resistance and mutagenesis. Other identified genes include those encoding DNA helicases (UvrD, RecG, RecQ), truncated forms of the DNA mismatch repair protein (MutS) and a primosomal component (DnaT), a negative modulator of initiation of replication/GATC-binding protein (SeqA), a stationary phase regulator AppY, a transcriptional regulator PaaX and three putative open reading frames, ycgW, yfjY and yjiD, encoding hypothetical...Continue Reading

References

Aug 27, 1975·Molecular & General Genetics : MGG·P Nevers, H C Spatz
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M L MichaelsJ H Miller
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S B FarrD Touati
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Y NghiemJ H Miller
Jul 1, 1987·Journal of Bacteriology·J A Imlay, S Linn
Jan 1, 1987·Journal of Cell Science. Supplement·S Linn, J A Imlay
Aug 14, 1970·Science·T C GibsonE C Cox
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H EcholsP M Burgers
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B W Glickman, M Radman
Jan 1, 1981·Journal of Bacteriology·G E Herman, P Modrich
Mar 1, 1981·Mutation Research·M D Gross, E C Siegel
May 1, 1995·Journal of Bacteriology·O LomovskayaA Matin
Sep 5, 1997·Science·F R BlattnerY Shao
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·N ShakibaiL Rothfield
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y CanitrotJ S Hoffmann
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·M TangM F Goodman
Oct 29, 2000·Journal of Bacteriology·M J Lombardo, S M Rosenberg
Jan 12, 2001·Molecular and Cellular Biology·P V ShcherbakovaT A Kunkel
May 22, 2001·Molecular Microbiology·A R Richardson, I Stojiljkovic
Dec 26, 2001·Journal of Bacteriology·Erick DenamurIvan Matic
Jun 5, 2002·Annual Review of Biochemistry·Shelley L Lusetti, Michael M Cox
Dec 24, 2002·Cancer Biology & Therapy·Christopher D HeinenRichard Fishel
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Lawrence A LoebJon P Anderson
Apr 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Anders Løbner-OlesenFlemming G Hansen
Aug 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Asa KarlssonDean W Felsher
Nov 1, 1954·Proceedings of the National Academy of Sciences of the United States of America·H P TreffersN O Belser

❮ Previous
Next ❯

Citations

Jan 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Houra MerrikhSusan T Lovett
May 9, 2012·Antimicrobial Agents and Chemotherapy·Muhammad MalikKarl Drlica
Dec 30, 2009·Applied and Environmental Microbiology·Ronnie MachielsenJohan E T Van Hylckama Vlieg
Oct 17, 2006·Journal of Bacteriology·Laurie H SandersMark D Sutton
Jul 5, 2005·Journal of Bacteriology·Vanessa GabrovskyJeffrey H Miller
Jun 10, 2006·Annual Review of Microbiology·John R RothDan I Andersson
Jul 27, 2012·Journal of Molecular Microbiology and Biotechnology·Jihwan HwangMasayori Inouye
Nov 28, 2009·Genome Biology·Jeffrey M SkerkerAdam P Arkin
Jan 23, 2016·Scientific Reports·Carlotta RondaAlex Toftgaard Nielsen
May 19, 2007·Metabolic Engineering·Yong-Su Jin, Gregory Stephanopoulos
Dec 2, 2008·Molecular Microbiology·Abu Amar M Al Mamun, M Zafri Humayun
Aug 2, 2006·Molecular Microbiology·Hanjing YangJeffrey H Miller
Oct 8, 2015·Nature Communications·Ahmed H Badran, David R Liu
Sep 11, 2007·FEMS Microbiology Letters·Juan-Carlos GalánFernando Baquero
May 27, 2005·Critical Reviews in Biochemistry and Molecular Biology·Jeffrey H Miller
Aug 23, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Susan M RosenbergP J Hastings
Aug 6, 2004·Nature·Susan M Rosenberg, P J Hastings
Aug 10, 2007·Critical Reviews in Biochemistry and Molecular Biology·Claude Saint-RufIvan Matic
Aug 21, 2019·Journal of Applied Microbiology·L Fernandez-GarciaT K Wood
Dec 1, 2011·EcoSal Plus·Regine Hengge

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.