Identification of N-acyl homoserine lactones produced by Gluconacetobacter diazotrophicus PAL5 cultured in complex and synthetic media

Archives of Microbiology
Carlos G Nieto-PeñalverLucía I C de Figueroa

Abstract

The endophytic diazotrophic Gluconacetobacter diazotrophicus PAL5 was originally isolated from sugarcane (Saccharum officinarum). The biological nitrogen fixation, phytohormones secretion, solubilization of mineral nutrients and phytopathogen antagonism allow its classification as a plant growth-promoting bacterium. The recent genomic sequence of PAL5 unveiled the presence of a quorum sensing (QS) system. QS are regulatory mechanisms that, through the production of signal molecules or autoinducers, permit a microbial population the regulation of the physiology in a coordinated manner. The most studied autoinducers in gram-negative bacteria are the N-acyl homoserine lactones (AHLs). The usage of biosensor strains evidenced the presence of AHL-like molecules in cultures of G. diazotrophicus PAL5 grown in complex and synthetic media. Analysis of AHLs performed by LC-APCI-MS permitted the identification of eight different signal molecules, including C6-, C8-, C10-, C12- and C14-HSL. Mass spectra confirmed that this diazotrophic strain also synthesizes autoinducers with carbonyl substitutions in the acyl chain. No differences in the profile of AHLs could be determined under both culture conditions. However, although the level of sho...Continue Reading

References

Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·P D ShawS K Farrand
Sep 18, 1997·Applied and Environmental Microbiology·T Jimenez-SalgadoJ Caballero-Mellado
Jan 25, 2002·Journal of Bacteriology·Joseph T ByersMartin Welch
Sep 5, 2002·Nature Reviews. Molecular Cell Biology·Clay Fuqua, E Peter Greenberg
Jan 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ulrike MathesiusWolfgang D Bauer
Jun 21, 2003·Clinical Chemistry·Thomas M Annesley
Nov 12, 2005·Chembiochem : a European Journal of Chemical Biology·Irene Wagner-DöblerStefan Schulz
Oct 27, 2006·FEMS Microbiology Ecology·Ludovic VialFlorence Wisniewski-Dyé
Sep 16, 2008·Research in Microbiology·Mickaël BoyerFlorence Wisniewski-Dyé
Aug 19, 2009·Annual Review of Genetics·Wai-Leung Ng, Bonnie L Bassler
Jan 12, 2010·Trends in Microbiology·Alan W DechoPieter T Visscher
Jul 1, 1994·World Journal of Microbiology & Biotechnology·V M ReisJ Döbereiner

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Citations

Jun 30, 2014·Antonie van Leeuwenhoek·Elisa V BertiniLucía I C de Figueroa
Jul 23, 2014·Research in Microbiology·Carlos G Nieto-PeñalverLucía I C de Figueroa
May 15, 2015·Applied Microbiology and Biotechnology·Parijat KusariOliver Kayser
May 19, 2016·Journal of Natural Products·Vanessa G A OlherArmando M Pomini
Feb 18, 2021·AMB Express·Xiaoman QiuHousheng Hong
Sep 15, 2020·Microbiological Research·Manisha PhourBernard R Glick

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