Identification of N-octylnortadalafil and its Stereoisomers in Dietary Supplements with Chiral Liquid Chromatography-Circular Dichroism

Chirality
Miho SakamotoTakako Moriyasu

Abstract

A direct chiral liquid chromatography-circular dichroism (LC-CD) method was developed for the simple and rapid identification of N-octylnortadalafil [(6R, 12aR)-6-(1,3-benzodioxol-5-yl)-2-octyl-2,3,6,7,12,12a-hexahydropyrazino[1',2':1,6]pyrido[3,4-b]indole-1,4-dione; RR-OTDF] and its stereoisomers in dietary supplements. Samples were extracted with methanol. Compounds were then separated by chiral LC-CD using Chiralcel OD-RH (4.6 × 150 mm, 5 µm) with 5 mM ammonium formate (pH 3)/0.1% formic acid in acetonitrile (95:5, v/v) mixture solution (mobile phase A) and 0.1% formic acid in acetonitrile (mobile phase B). The isocratic elution used was mobile phase A / mobile phase B (3:7, v/v) at a flow rate of 1 ml/min. The column temperature was held at 30°C. RR-OTDF and its stereoisomers were separated within 20 min with the resolution factors being over 2.0. Using this method, RR-OTDF and (6R, 12aS)-6-(1,3-benzodioxol-5-yl)-2-octyl-2,3,6,7,12,12a-hexahydropyrazino[1',2':1,6]pyrido[3,4-b]indole-1,4-dione were detected in a dietary supplement.

References

Apr 29, 2006·Food Additives and Contaminants·Peng ZouHwee-Ling Koh
Nov 21, 2007·Journal of Chromatographic Science·Wenhui GaoGuijian Liang
Sep 13, 2008·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Takashi HasegawaYukihiro Goda
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Nov 8, 2011·Journal of Pharmaceutical and Biomedical Analysis·Valerie M ToomeyCheryl L Flurer
Feb 20, 2013·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Eung-Sun LeeJinho Kim
Jun 1, 2013·Journal of Pharmaceutical and Biomedical Analysis·Dhavalkumar Narendrabhai PatelHwee-Ling Koh

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Citations

Sep 3, 2019·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Miho SakamotoKoichi Saito

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