Identification of naphthalene carboxylase as a prototype for the anaerobic activation of non-substituted aromatic hydrocarbons

Environmental Microbiology
Housna MouttakiRainer U Meckenstock

Abstract

Polycyclic aromatic hydrocarbons such as naphthalene are recalcitrant environmental pollutants that are only slowly metabolized by bacteria under anoxic conditions. Based on metabolite analyses of culture supernatants, carboxylation or methylation of naphthalene have been proposed as initial enzymatic activation reactions in the pathway. However, the extremely slow growth of anaerobic naphthalene degraders with doubling times of weeks and the little biomass obtained from such cultures hindered the biochemical elucidation of the initial activation reaction, so far. Here, we provide biochemical evidence that anaerobic naphthalene degradation is initiated via carboxylation. Crude cell extracts of the sulfate-reducing enrichment culture N47 converted naphthalene and (13)C-labelled bicarbonate to 2-[carboxyl-(13)C]naphthoic acid at a rate of 0.12 nmol min(-1) mg protein(-1) . The enzyme, namely naphthalene carboxylase, catalysed a much faster exchange of (13) C-labelled bicarbonate with the carboxyl group of 2-[carboxyl-(12)C]naphthoic acid at a rate of 3.2 nmol min(-1) mg protein(-1), indicating that the rate limiting step of the carboxylation reaction is the activation of the naphthalene molecule rather than the carboxylation itse...Continue Reading

References

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Citations

Feb 9, 2016·Environmental Science & Technology·Steffen KümmelCarsten Vogt
Jan 23, 2013·Environmental Microbiology·Christian EberleinRainer U Meckenstock
Nov 27, 2014·Molecular Microbiology·R Gary Sawers
Sep 25, 2012·Current Opinion in Biotechnology·Amy V Callaghan
Mar 13, 2015·FEMS Microbiology Ecology·Steffen KümmelCarsten Vogt
Aug 19, 2014·Frontiers in Microbiology·Andreas TeskeDouglas E LaRowe
Mar 25, 2014·FEMS Microbiology Letters·Brandon E L MorrisMatthias Boll
Jan 20, 2017·International Journal of Systematic and Evolutionary Microbiology·Janina S KoelschbachRainer U Meckenstock
Jun 3, 2017·Environmental Microbiology·Philip WeyrauchRainer U Meckenstock
Jun 23, 2018·Chemistry : a European Journal·Max WillisteinMatthias Boll
Jul 28, 2018·Environmental Microbiology·Anne M HimmelbergRainer U Meckenstock
Aug 6, 2019·Advanced Synthesis & Catalysis·Stefan E PayerSilvia M Glueck
Mar 17, 2019·Biodegradation·J S KoelschbachR U Meckenstock
Feb 26, 2021·ChemSusChem·Godwin A AlekuDavid Leys

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