Identification of native rat cerebellar granule cell currents due to background K channel KCNK5 (TASK-2)

Brain Research. Molecular Brain Research
Joseph F CottenC Spencer Yost

Abstract

The TWIK-related, Acid Sensing K (TASK-2; KCNK5) potassium channel is a member of the tandem pore (2P) family of potassium channels and mediates an alkaline pH-activated, acid pH-inhibited, outward-rectified potassium conductance. In previous work, we demonstrated TASK-2 protein expression in newborn rat cerebellar granule neurons (CGNs). In this study, we demonstrate TASK-2 functional expression in CGNs as a component of the pH-sensitive, volatile anesthetic-potentiated, standing-outward potassium conductance (I(K,SO)). Using excised, inside-out patch-clamp technique, we studied CGNs grown in primary culture. We identified four distinct, noninactivating single channel potassium conductances, Types 1-4. Types 1-3 have previously been attributed to TASK-1 (KCNK3), TASK-3 (KCNK9) and TASK-1/TASK-3 heteromers, and TREK-2 (KCNK10) 2P potassium channel function, respectively; however, the Type 4 conductance is currently unassigned. Previous studies demonstrated that Type 4 single channel activity is potentiated by extracellular, alkaline pH and cytoplasmic arachidonic acid (10-20 microM) and inhibited by cytoplasmic tetraethylammonium (TEA; 1 mM). We determined that heterologously expressed TASK-2 channels have single channel gating...Continue Reading

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Citations

Apr 18, 2013·PloS One·Brian M Nadin, Paul J Pfaffinger
Jun 24, 2008·Behavioural Brain Research·C Spencer YostJames M Sonner
Feb 10, 2006·Biochemical and Biophysical Research Communications·Dawon KangJaehee Han
Sep 20, 2005·Biochemical and Biophysical Research Communications·Stephen P BrazierPaul J Kemp
Nov 12, 2013·Neuropharmacology·R Noriega-NavarroJ Garcia-Valdes
Aug 22, 2013·Channels·Mikio Hayashi, Ivana Novak
Oct 16, 2014·Pflügers Archiv : European journal of physiology·Eva GöbChristoph Kleinschnitz
Feb 11, 2011·American Journal of Physiology. Cell Physiology·Joo Hyun NamSung Joon Kim

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