PMID: 1675221May 1, 1991

Identification of Neisseria gonorrhoeae using the Neisstrip rapid enzyme detection test

Journal of Clinical Pathology
S F DeallerP M Hawkey

Abstract

A rapid enzyme activity strip test (Neisstrip, Lab M Ltd, Bury) was compared retrospectively with Phadebact Monoclonal GC coagglutination (Pharmacia Diagnostics, Uppsala, Sweden), cystine trypticase agar sugar utilisation (CTA), and Gonochek II (J W Turner, Liverpool) enzyme methods for identification of 95 Neisseria spp and related species. These had been previously identified using standard methods and included 29 that had given aberrant results. Neisstrip identified correctly all but two, including nine incorrectly identified by Phadebact and 18 erroneously identified using CTA sugars. Results were similar to those obtained with Gonochek II. After this a prospective study was performed testing 400 oxidase positive isolates derived from clinical samples cultured on gonococcal selective medium. Two organisms, both Moraxella spp, were incorrectly identified as N gonorrhoeae by the Neisstrip. The superoxol test, when used with either the Phadebact or Neisstrip tests, maintained 100% sensitivity and specificity. The Neisstrip is a rapid, economic test that is accurate and easy to interpret. It may be used alone or in conjunction with a superoxol test or a coagglutination test, which is relatively accurate but more expensive, and ...Continue Reading

References

Oct 1, 1988·Clinical Microbiology Reviews·J S Knapp
Feb 1, 1988·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·W M Janda, V Sobieski
Apr 1, 1984·The British Journal of Venereal Diseases·H YoungJ Tapsall

Citations

Jul 28, 2010·Sexually Transmitted Diseases·Irene E MartinLai-King Ng
Aug 1, 2006·Sexually Transmitted Infections·S AlexanderCatherine A Ison
Sep 15, 2006·Sexually Transmitted Infections·L OteroF Vázquez
Jun 27, 2009·Journal of Industrial Microbiology & Biotechnology·Jaume Comas-Riu, Núria Rius
Jan 1, 1997·Applied Neuropsychology·R A RuchinskasJ T Barth
Nov 7, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Elizabeth G BromfieldBrett Nixon

Related Concepts

Proline iminopeptidase
Aminopeptidase
Bacteriological Techniques
Lactogest
Gamma-glutamyl transferase
Gonorrhea
Neisseria gonorrhoeae
Reagent Strips
Retrospective Studies

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