Identification of neutralization and diagnostic epitopes on PIM, the polymorphic immunodominant molecule of Theileria parva.

Infection and Immunity
P ToyeA J Musoke

Abstract

The polymorphic immunodominant molecule (PIM) of Theileria parva is expressed by the schizont and sporozoite stages of the parasite. We have recently cloned the cDNA encoding the PIM antigen from two stocks of the parasite: the cattle-derived T. parva (Muguga) stock and a buffalo-derived stock. The cDNAs were used in transient-transfection assays to assess the reactivity of the antigen with monoclonal antibodies (MAb) previously raised against schizont-infected cells and used for parasite strain identification. We demonstrate that 19 of the 25 MAb are specific for PIM. Antibody reactivities with deletion mutants of a fusion protein containing PIM and Pepscan analysis of the Muguga version of the molecule with 13 of the MAb indicate that there are at least 10 different epitopes throughout the molecule. None of the MAb react with a tetrapeptide repeat present in the central region of the molecule, probably because of an inability of BALB/c mice to produce antibodies to this repeat. In contrast, sera from infected cattle react strongly with the repeat region, suggesting that this region alone may be useful as a diagnostic reagent. Previous studies showed that MAb to PIM inhibit sporozoite infectivity of bovine lymphocytes in vitro...Continue Reading

References

Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A MusokeV Nene
Jan 1, 1987·Annual Review of Microbiology·D J KempR F Anders
Jan 1, 1980·Journal of Immunological Methods·T W PearsonR S Hwett

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Citations

Sep 21, 2016·Parasite Immunology·V Nene, W I Morrison
Oct 24, 2007·PLoS Pathogens·Kelly A BraytonVishvanath M Nene
May 22, 2019·Frontiers in Immunology·Reginaldo G BastosLindsay M Fry
Mar 15, 2016·Ticks and Tick-borne Diseases·Vishvanath NeneLucilla Steinaa
May 12, 2004·Veterinary Parasitology·G O MateteS G Ndung'u

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