Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain

Journal of Virology
M OstrowskiO J Lopez

Abstract

After infection of swine with porcine reproductive and respiratory syndrome virus (PRRSV), there is a rapid rise of PRRSV-specific nonneutralizing antibodies (NNA), while neutralizing antibodies (NA) are detectable not sooner than 3 weeks later. To characterize neutralizing epitopes, we selected phages from a 12-mer phage display library using anti-PRRSV neutralizing monoclonal antibody (MAb) ISU25-C1. In addition, phages carrying peptides recognized by swine antibodies with high seroneutralizing titer were isolated after subtracting from the library those clones binding to swine anti-PRRSV serum with no neutralizing activity. Two epitopes located in the ectodomain of PRRSV GP5 were identified. One of these epitopes, which we named epitope B, was recognized both by neutralizing MAb ISU25-C1 and swine neutralizing serum (NS) but not by swine nonneutralizing serum (NNS), indicating that it is a neutralizing epitope. Epitope B is sequential, conserved among isolates, and not immunodominant. Antibodies directed against it are detected in serum late after infection. In contrast, the other epitope, which we named epitope A, is hypervariable and immunodominant. Antibodies against it appear early after infection with PRRSV. This epitop...Continue Reading

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Citations

Jul 24, 2010·Virus Genes·Jie SongBaohua Zhao
Jun 22, 2012·Animal Health Research Reviews·Gourapura J RenukaradhyaDavid Benfield
Jan 7, 2003·Viral Immunology·Michael P MurtaughFederico Zuckermann
Apr 15, 2008·Transboundary and Emerging Diseases·Y-J ZhouX-F Yuan
Feb 11, 2012·Clinical and Vaccine Immunology : CVI·Steven M SzczepanekLawrence K Silbart
Mar 25, 2011·Clinical and Vaccine Immunology : CVI·Benjamin R TribleRaymond R R Rowland
Oct 19, 2007·Journal of Virology·Prasad MulupuriMichael P Murtaugh
Dec 13, 2002·Journal of Virology·Eric J SnijderWilly J M Spaan
Jul 16, 2004·Journal of Virology·Peter L Delputte, Hans J Nauwynck

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