Identification of Neutralizing Monoclonal Antibodies Targeting Novel Conformational Epitopes of the Porcine Epidemic Diarrhoea Virus Spike Protein

Scientific Reports
Chia-Yu ChangHui-Wen Chang

Abstract

Since 2010, newly identified variants of porcine epidemic diarrhoea virus (PEDV) have caused high mortality in neonatal piglets which has devastated the swine industry. The spike (S) glycoprotein of PEDV contains multiple neutralizing epitopes and is a major target for PEDV neutralization and vaccine development. To understand the antigenicity of the new PEDV variant, we characterized the neutralizing epitopes of a new genotype 2b PEDV isolate from Taiwan, PEDV Pintung 52 (PEDV-PT), by the generation of neutralizing monoclonal antibodies (NmAbs). Two NmAbs, P4B-1, and E10E-1-10 that recognized the ectodomain of the full-length recombinant PEDV S protein and exhibited neutralizing ability against the PEDV-PT virus were selected. Recombinant truncated S proteins were used to identify the target sequences for the NmAbs and P4B-1 was shown to recognize the C-terminus of CO-26K equivalent epitope (COE) at amino acids (a.a.) 575-639 of the PEDV S. Interestingly, E10E-1-10 could recognize a novel neutralizing epitope at a.a. 435-485 within the S1A domain of the PEDV S protein, whose importance and function are yet to be determined. Moreover, both NmAbs could not bind to linearized S proteins, indicating that only conformational epitop...Continue Reading

References

Jan 1, 1978·Archives of Virology·M B Pensaert, P de Bouck
Aug 21, 1986·Nature·D J BarlowJ M Thornton
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Sep 27, 2006·Protein Science : a Publication of the Protein Society·Pernille Haste AndersenOle Lund
Apr 11, 2008·Veterinary Microbiology·Dongbo SunGuangzhi Tong
Oct 24, 2012·The Journal of Biological Chemistry·Guiqing PengFang Li
Aug 22, 2013·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Gregory W StevensonKyoungjin J Yoon
Apr 23, 2014·Emerging Infectious Diseases·Leyi WangYan Zhang
Jun 25, 2014·Emerging Infectious Diseases·Sunhee Lee, Changhee Lee
Mar 20, 2015·Journal of Virology·Chang LiuFang Li
Apr 22, 2015·Archives of Virology·Chang-chao HuanXiang Mao
Nov 6, 2015·The Journal of Veterinary Medical Science·Dongbo SunLi Feng
Dec 8, 2015·Journal of Animal Science·Lee L Schulz, Glynn T Tonsor
Jun 19, 2016·Virus Research·Wentao LiBerend-Jan Bosch
Sep 13, 2016·Nature Structural & Molecular Biology·Alexandra C WallsDavid Veesler

❮ Previous
Next ❯

Citations

Jan 23, 2020·International Journal of Molecular Sciences·Jiayu FuXiaobo Huang
Nov 27, 2020·Microorganisms·Souvik Ghosh, Yashpal S Malik
Nov 7, 2020·Transboundary and Emerging Diseases·Mingpu QiDouglas G Marthaler

❮ Previous
Next ❯

Datasets Mentioned

BETA
KY929405.1

Methods Mentioned

BETA
ELISAs
ELISA
dot-blot
protein folding
phage-display
Protein Assay
electrophoresis
enzyme-linked immunosorbent assay

Software Mentioned

UCSF ChimeraX
SWISS Model
UCSF ChimeraX system

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.