Identification of new localized RNAs in the Xenopus oocyte by differential display PCR

Developmental Genetics
J W HudsonR P Elinson

Abstract

We have identified localized transcripts in full-grown Xenopus oocytes by differential display PCR. One clone, An4a, has two transcripts, which localize to the animal half of the stage VI oocyte. The transcripts are expressed throughout early development, with embryonic expression primarily in anterior neural tissues. An4a has a high degree of sequence identity to a human cDNA clone of unknown function. Another clone, the previously identified beta-transducin repeat containing protein (beta-TrCP), has three transcripts with a unique pattern of localization, one localized to the animal half and two localized primarily to the vegetal cortex. This cDNA has previously been shown to rescue a yeast cell division cycle mutant, raising the possibility that the different Xenopus transcripts are involved in animal and vegetal cell cycles. Embryonic expression is primarily in the cement gland. These new localized transcripts contribute to the general observation that the vegetal cortex, but not the animal cortex, is a specific site for RNA localization.

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Citations

May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·C LiuX He
Sep 3, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M L KingM Bubunenko
Jan 1, 1996·Developmental Genetics·M L King
Jun 20, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Fiona C Wardle, Hazel L Sive
Oct 25, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Paaqua A GrantSally A Moody
Dec 17, 2004·Biology of the Cell·Mary Lou KingKimberly L Mowry
May 27, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tawny N Cuykendall, Douglas W Houston
Apr 22, 1999·Microscopy Research and Technique·P ChangE Houliston
Mar 4, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K L Mowry, C A Cote
Aug 14, 1999·Mechanisms of Development·D GradlD Wedlich
Jul 27, 2002·Developmental Biology·Mikhail BubunenkoMary Lou King
Oct 6, 1998·Annual Review of Biochemistry·A BashirullahH D Lipshitz

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