Identification of new phosphorylation sites of CD23 in B-cells of patients with chronic lymphocytic leukemia

Leukemia Research
Martina MaďarováTomas Dobransky

Abstract

B-cell chronic lymphocytic leukemia (B-CLL) is the most common lymphoproliferative disorder in adults. Patients with B-CLL strongly express the CD23 - C type of lectin (low affinity IgE receptor, Fc epsilon RII), which is linked to B cell activation and proliferation. Phosphorylation in lymphocytes is tightly associated with regulation of protein activities, functional regulation and cell signaling, and may thus affect initiation and/or progression of the disease. Here we report changes in the phosphorylation of CD23 on threonine (pThr314) and two serine residues (pSer254, pSer265) in B lymphocytes of B-CLL patients, using a flow cytometry approach. The majority of tested patients with active forms of B-CLL presented a notable overexpression of CD23 along with pThr314, pSer254, and pSer265 CD23 phosphorylation positivity. Moreover, we have experimentally stimulated the CD23 phosphorylations in a subset of peripheral blood lymphocytes of healthy controls by phorbol-12-myristate-13-acetate treatment. This affects the activation of competent phosphorylation mediating kinases, resulting in the enhanced phosphorylation pattern. Together, these data confirm that CD23 protein is phosphorylated in B cells of B-CLL patients, report the ...Continue Reading

References

Jan 1, 1992·Immunologic Research·M SarfatiG Delespesse
Apr 1, 1992·The Journal of Clinical Investigation·S FournierM Sarfati
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A YokotaH Kikutani
May 1, 1989·Immunology Today·G DelespesseH Hofstetter
Feb 1, 1995·British Journal of Haematology·S FournierM Sarfati
May 1, 1996·European Journal of Immunology·E L BurlinsonW Cushley
Jan 1, 1997·International Reviews of Immunology·J Y BonnefoyC Plater-Zyberk
Aug 24, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·F Caligaris-Cappio, T J Hamblin
Jul 20, 2002·Leukemia Research·Martin E GollerHans Peter Tony
Sep 25, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Peter O Krutzik, Garry P Nolan
Sep 2, 2010·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Marcia A ChanLanny J Rosenwasser
Sep 14, 2010·Clinical and Experimental Immunology·M AcharyaW Cushley
Jul 5, 2011·ACS Chemical Biology·Heather L Rust, Paul R Thompson
Oct 2, 2012·The Journal of Clinical Investigation·Gianluca GaidanoRiccardo Dalla-Favera
Oct 9, 2014·BioMed Research International·Christina KalpadakisMaria K Angelopoulou

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Citations

May 7, 2019·Frontiers in Immunology·Sanae Ben MkaddemRenato C Monteiro
May 25, 2020·European Journal of Applied Physiology·Franz-Xaver ReichlBurkhard Summer

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