Identification of New Regulators of the Oocyte-to-Embryo Transition in Drosophila.

G3 : Genes - Genomes - Genetics
Emir E Avilés-PagánTerry L Orr-Weaver

Abstract

At the oocyte-to-embryo transition the highly differentiated oocyte arrested in meiosis becomes a totipotent embryo capable of embryogenesis. Oocyte maturation (release of the prophase I primary arrest) and egg activation (release from the secondary meiotic arrest and the trigger for the oocyte-to-embryo transition) serve as prerequisites for this transition, both events being controlled posttranscriptionally. Recently, we obtained a comprehensive list of proteins whose levels are developmentally regulated during these events via a high-throughput quantitative proteomic analysis of Drosophila melanogaster oocyte maturation and egg activation. We conducted a targeted screen for potential novel regulators of the oocyte-to-embryo transition, selecting 53 candidates from these proteins. We reduced the function of each candidate gene using transposable element insertion alleles and RNAi, and screened for defects in oocyte maturation or early embryogenesis. Deletion of the aquaporin gene CG7777 did not affect female fertility. However, we identified CG5003 and nebu (CG10960) as new regulators of the transition from oocyte to embryo. Mutations in CG5003, which encodes an F-box protein associated with SCF-proteasome degradation functio...Continue Reading

References

May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Mar 15, 1997·Developmental Biology·A W Page, T L Orr-Weaver
Sep 27, 2002·Genesis : the Journal of Genetics and Development·Torsten BossingAndrea H Brand
May 17, 2003·Pflügers Archiv : European journal of physiology·Marc Uldry, Bernard Thorens
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Matthew D Petroski, Raymond J Deshaies
Feb 12, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Vanessa L Horner, Mariana F Wolfner
Apr 5, 2012·American Journal of Physiology. Endocrinology and Metabolism·Qiang WangKelle H Moley
Dec 12, 2012·Cell Calcium·Caroline V Sartain, Mariana F Wolfner
Mar 20, 2013·Molecular Aspects of Medicine·Mike Mueckler, Bernard Thorens
May 10, 2013·Nature Reviews. Molecular Cell Biology·Jeffrey R SkaarMichele Pagano
Jun 25, 2013·Genetics·Amber R KrauchunasMariana F Wolfner
Mar 14, 2014·G3 : Genes - Genomes - Genetics·Jason M TennessenCarl S Thummel
Mar 19, 2014·Methods : a Companion to Methods in Enzymology·Jason M TennessenCarl S Thummel
Oct 29, 2014·Proceedings of the National Academy of Sciences of the United States of America·Iva KronjaTerry L Orr-Weaver
Oct 18, 2016·Trends in Cell Biology·María Salazar-Roa, Marcos Malumbres
Dec 16, 2017·Seminars in Cell & Developmental Biology·Gabriela Huelgas-Morales, David Greenstein
Feb 16, 2018·Seminars in Cell & Developmental Biology·Emir E Avilés-Pagán, Terry L Orr-Weaver
Feb 21, 2018·Biology of Reproduction·Richard M SchultzPetr Svoboda
Aug 8, 2018·Proceedings of the National Academy of Sciences of the United States of America·Boryana PetrovaTerry L Orr-Weaver
Oct 27, 2018·Nucleic Acids Research·Jim ThurmondUNKNOWN FlyBase Consortium
Mar 29, 2019·Development·Takayuki YamadaTakashi Nishimura

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Methods Mentioned

BETA
dissection
Transgenic
PCR
protein assay
ubiquitination

Software Mentioned

Excel

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