Identification of Nilaparvata lugens and Its Two Sibling Species (N. bakeri and N. muiri) by Direct Multiplex PCR

Journal of Economic Entomology
Shuhua LiuJian Tang

Abstract

The brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is a destructive rice pest of Asia. Currently, one important monitoring method of BPH is through black light trapping. However, two sibling species of BPH, Nilaparvata bakeri (Muri) and Nilaparvata muiri China, can also be trapped by black light, and these species feed only on gramineous weeds rather than on rice. Therefore, the accurate identification of Nilaparvata species is crucial for N. lugens forecasting and management. The traditional morphological identification method is not feasible for subadults and damaged specimens. Furthermore, this error-prone morphological identification method is time and labor intensive, with the need for expertise and experience. Here, we established a direct multiplex polymerase chain reaction (dmPCR) assay using crude tissue fluid as a template, omitting purified DNA extraction. The crude tissue fluid can be obtained by grinding specimens without any biological reagent but only using distilled water. This dmPCR assay, using three pairs of diagnostic primers, is based on internal transcribed spacers (ITS). Each primer pair amplifies a species-specific fragment of a different size, which were easily and reliably...Continue Reading

References

Jul 22, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·C BrenguesV Robert
Mar 18, 2016·Annual Review of Entomology·Christian Nansen, Norman Elliott
Nov 20, 2016·Ticks and Tick-borne Diseases·Kairi VärvIrina Golovljova

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.