Identification of nitroxyl-induced modifications in human platelet proteins using a novel mass spectrometric detection method.

Molecular & Cellular Proteomics : MCP
Michael D HoffmanJuergen Kast

Abstract

Nitroxyl (HNO) exhibits many important pharmacological effects, including inhibition of platelet aggregation, and the HNO donor Angeli's salt has been proposed as a potential therapeutic agent in the treatment of many diseases including heart failure and alcoholism. Despite this, little is known about the mechanism of action of HNO, and its effects are rarely linked to specific protein targets of HNO or to the actual chemical changes that proteins undergo when in contact with HNO. Here we study the presumed major molecular target of HNO within the body: protein thiols. Cysteine-containing tryptic peptides were reacted with HNO, generating the sulfinamide modification and, to a lesser extent, disulfide linkages with no other long lived intermediates or side products. The sulfinamide modification was subjected to a comprehensive tandem mass spectrometric analysis including MS/MS by CID and electron capture dissociation as well as an MS(3) analysis. These studies revealed a characteristic neutral loss of HS(O)NH2 (65 Da) that is liberated from the modified cysteine upon CID and can be monitored by mass spectrometry. Upon storage, partial conversion of the sulfinamide to sulfinic acid was observed, leading to coinciding neutral los...Continue Reading

References

Oct 2, 1992·Journal of Medicinal Chemistry·M J LeeE G DeMaster
Feb 1, 1989·The Journal of Cell Biology·J H HartwigT P Stossel
Dec 1, 1987·International Journal of Peptide and Protein Research·S Clarke
Nov 15, 1996·Archives of Biochemistry and Biophysics·K D GreisS Matalon
Mar 21, 1998·Archives of Biochemistry and Biophysics·D A WinkJ B Mitchell
Jun 17, 2000·Analytical Chemistry·Z Y Park, D H Russell
Aug 29, 2000·Journal of Muscle Research and Cell Motility·I Dalle-DonneR Rossi
Sep 20, 2000·The Journal of Biological Chemistry·B Yan, J W Smith
Oct 24, 2000·The Journal of Biological Chemistry·K M MirandaD A Wink
Apr 27, 2001·Journal of the American Society for Mass Spectrometry·A SarverB W Gibson
Jul 4, 2001·Journal of Mass Spectrometry : JMS·A S PeterssonP Roepstorff
Jul 27, 2001·Biochemistry·B Yan, J W Smith
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·N PaolocciD A Kass
Sep 13, 2001·Journal of Neurochemistry·C A ColtonS Vicini
Oct 31, 2001·The Journal of Biological Chemistry·J WangP B Chock
Sep 5, 2002·Free Radical Biology & Medicine·Michael Graham EspeyDavid A Wink
Dec 12, 2002·Mass Spectrometry Reviews·Jeffrey J GormanJames J Pitt
Dec 25, 2002·Free Radical Biology & Medicine·Pasquale PagliaroNazareno Paolocci
Apr 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Martin Feelisch
Apr 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nazareno PaolocciDavid A Kass
May 3, 2003·Trends in Molecular Medicine·Matthew W FosterJonathan S Stamler
May 22, 2003·The International Journal of Biochemistry & Cell Biology·R K AndrewsM C Berndt
Jul 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Katrina M MirandaDavid A Wink
Dec 3, 2003·Molecular & Cellular Proteomics : MCP·J P McRedmondD J Fitzgerald
Mar 9, 2004·Molecular Biology of the Cell·Enni BertlingPekka Lappalainen
Apr 23, 2004·Current Opinion in Biotechnology·Roman A Zubarev
Dec 28, 2004·Chembiochem : a European Journal of Chemical Biology·Jon M FukutoKendall N Houk
Jan 13, 2005·Journal of the American Chemical Society·Katrina M MirandaDavid A Wink
Feb 5, 2005·Toxicology·Harry Ischiropoulos, Andrew Gow
Sep 6, 2005·Archives of Biochemistry and Biophysics·Brenda E LopezJon M Fukuto
Oct 26, 2005·Biochemical Society Transactions·T R HurdM P Murphy
Nov 5, 2005·Thrombosis and Haemostasis·Emilse BermejoMaría A Lazzari

❮ Previous
Next ❯

Citations

Aug 3, 2010·Antioxidants & Redox Signaling·Michelle L BullenBarbara K Kemp-Harper
Jan 18, 2011·Antioxidants & Redox Signaling·Wilmarie Flores-SantanaDavid A Wink
Jan 18, 2011·Antioxidants & Redox Signaling·Jon M Fukuto, Samantha J Carrington
Jan 18, 2011·Antioxidants & Redox Signaling·Carlo G TocchettiNazareno Paolocci
Feb 9, 2011·Antioxidants & Redox Signaling·Barbara K Kemp-Harper
Mar 17, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Nazareno Paolocci, David A Wink
Apr 23, 2013·Journal of Proteomics·Helena RyšlaváOndřej Vaněk
Jul 17, 2010·Journal of Biomedicine & Biotechnology·Cordula Klockenbusch, Juergen Kast
Apr 20, 2010·Amino Acids·Corinne M Spickett, Andrew R Pitt
Oct 1, 2010·Trends in Cardiovascular Medicine·Nicoleta AlexandruAdriana Georgescu
Jun 29, 2015·Free Radical Biology & Medicine·Douglas D ThomasKatrina M Miranda
May 12, 2009·Biochimica Et Biophysica Acta·Christopher H SwitzerDavid A Wink
May 30, 2014·Biochemistry·Gizem Keceli, John P Toscano
May 11, 2012·Biochemistry·Gizem Keceli, John P Toscano
Oct 1, 2013·Biochemistry·Gizem KeceliJohn P Toscano
Aug 26, 2014·Molecular & Cellular Proteomics : MCP·Cordula KlockenbuschJuergen Kast
Mar 29, 2014·Circulation Research·Julia M BurkhartRené P Zahedi
Mar 8, 2019·The Journal of General Physiology·Gizem KeceliJohn P Toscano
Jul 8, 2020·International Journal of Molecular Sciences·Erica GianazzaElena Tremoli
Jan 16, 2016·Oxidative Medicine and Cellular Longevity·Mai E Shoman, Omar M Aly
Aug 7, 2010·Analytical Chemistry·Miao LiuShi-Jian Ding
Jan 25, 2018·Journal of Natural Products·Janusz J PetkowskiSara Seager

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.