Identification of non-peptide species in selenized yeast by MALDI mass spectrometry using post-source decay and orthogonal Q-TOF detection

The Analyst
Jorge Ruiz EncinarRyszard Łobinski

Abstract

The potential of tandem mass spectrometry following matrix-assisted laser desorption ionization (MALDI) was studied for speciation of selenium. Non-peptide selenium-containing compounds were isolated from a selenized yeast aqueous extract by size-exclusion chromatography. Post-source decay (PSD) was compared with orthogonal quadrupole collision cell dissociation for the purpose of obtaining fragmentation and structural information. In the PSD mode, the use of ion gate covering the whole isotopic cluster of the parent compound allowed the immediate recognition of fragments containing Se and those in which this element was absent. The tandem mass spectra obtained by orthogonal MALDI Q-TOF were equally informative in terms of the number of fragments but suffered from a poorer sensitivity. The mass accuracy was ca. 20 times better in the oMALDI configuration than in the PSD mode. An unknown selenium compound with an m/z 388 was detected with a mass accuracy of 3 ppm according to the proposed empiric formula.

References

Feb 1, 1996·Journal of Inorganic Biochemistry·M A BeilsteinP D Whanger
Sep 7, 2000·Current Opinion in Biotechnology·M J Chalmers, S J Gaskell
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Citations

Apr 21, 2005·Analytical and Bioanalytical Chemistry·Heidi Goenaga InfanteTim Catterick
May 23, 2006·Analytical and Bioanalytical Chemistry·Alfred V Hirner
Jul 11, 2006·Analytical and Bioanalytical Chemistry·Emmie DumontRita Cornelis
Nov 8, 2005·Mass Spectrometry Reviews·Ryszard LobińskiJoanna Szpunar
Oct 31, 2007·Journal of Agricultural and Food Chemistry·Adriana AlzateCarmen Cámara

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