PMID: 1068458Oct 1, 1976

Identification of nonhistone chromatin proteins in chromatin subunits

Proceedings of the National Academy of Sciences of the United States of America
C C Liew, P K Chan

Abstract

Rat liver chromatin was digested by micrococcal nuclease. More than 80% of the enzyme-digested chromatin could be recovered after centrifugation. Treatment with sodium deoxycholate and Triton X-100 at concentrations of 0.5% in the final chromatin suspension gave a higher recovery. Chromatin subunits were fractionated on a 5-30% linear sucrose density gradient. Approximately 35% of the chromatin subunits could be recovered from the gradient. Chromatin subunits and their DNA fragments were identified by gel electrophoresis and ultracentrifugation. The presence of nonhistone chromatin proteins (NHCP) in chromatin subunits was demonstrated by the following criteria: (i) Quantitative analysis showed that the mass ratio of histone to NHCP, in the presence or absence of detergents, was 1:0,25 or 1:0.1, respectively. (ii) After the removal of acid-soluble protein from the subunits, it was found that most of the phenol-soluble NHCP were similar to total chromatin NHCP. However, four major fractions of these phenol-soluble NHCP were found to be enriched in the subunits as identified by two-dimensional polyacrylamide gel electrophoresis. (iii) Experiments using an exchange of isotope-labeled and nonlabeled chromatin showed that NHCP were ...Continue Reading

References

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Citations

Apr 21, 1993·Molecular and Cellular Biochemistry·C C Liew, E Cukerman
Aug 2, 1977·Biochimica Et Biophysica Acta·I BrownN Straus
Aug 1, 1985·Journal of Steroid Biochemistry·A GeierB Lunenfeld
May 1, 1986·Journal of Steroid Biochemistry·A GeierB Lunenfeld
Jun 1, 1987·Journal of Steroid Biochemistry·Y H XuT Y Wang
Jul 1, 1979·Journal of the Neurological Sciences·I H FraserC C Liew
Dec 15, 1977·The New England Journal of Medicine·A W Nienhuis, E J Benz
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·I L GoldknopfH Busch
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P C HentzenI Bekhor
Jul 1, 1980·The Journal of Cell Biology·K E Peters, D E Commings
Jan 1, 1980·Chemico-biological Interactions·M Gronow
Aug 1, 1978·Analytical Biochemistry·C Krüger, K Pollow
Aug 4, 1981·Biochemistry·D P QuickJ A Duerre
Nov 2, 1978·European Journal of Biochemistry·V V BakayevG P Georgiev
Aug 1, 1978·European Journal of Biochemistry·N DeferJ Kruh
Mar 1, 1978·European Journal of Biochemistry·E J SchlaegerR Knippers
Aug 15, 1977·European Journal of Biochemistry·J BöhmR Knippers
Jul 11, 2015·Clinical Epigenetics·Christian BuschSascha Venturelli

Related Concepts

Chromatin
Chromosomal Proteins, Non-Histone
SDS-PAGE
Liver
Plasma Protein Binding Capacity

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