Identification of nonsteroidal anti-inflammatory drug-activated gene (NAG-1) as a novel downstream target of phosphatidylinositol 3-kinase/AKT/GSK-3beta pathway

The Journal of Biological Chemistry
Kiyoshi YamaguchiSeung Joon Baek

Abstract

The signaling pathway of phosphatidylinositol 3-kinase (PI3K)/AKT, which is involved in cell survival, proliferation, and growth, has become a major focus in targeting cancer therapeutics. Nonsteroidal anti-inflammatory drug-activated gene (NAG-1) was previously identified as a gene induced by several anti-tumorigenic compounds including nonsteroidal anti-inflammatory drugs, peroxisome proliferator-activated receptor gamma ligands, and dietary compounds. NAG-1 has been shown to exhibit anti-tumorigenic and/or pro-apoptotic activities in vivo and in vitro. In this report, we showed a PI3K/AKT/glycogen synthase kinase-3beta (GSK-3beta) pathway regulates NAG-1 expression in human colorectal cancer cells as assessed by the inhibition of PI3K, AKT, and GSK-3beta. PI3K inhibition by LY294002 showed an increase in NAG-1 protein and mRNA expression, and 1l-6-hydroxymethyl-chiro-inositol 2(R)-2-O-methyl-3-O-octadecylcarbonate (AKT inhibitor) also induced NAG-1 expression. LY294002 caused increased apoptosis, cell cycle, and cell growth arrest in HCT-116 cells. Inhibition of GSK-3beta, which is negatively regulated by AKT, using AR-A014418 and lithium chloride completely abolished LY294002-induced NAG-1 expression as well as the NAG-1 pr...Continue Reading

References

Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·S RocheS A Courtneidge
Aug 1, 1998·The Journal of Biological Chemistry·M Pap, G M Cooper
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·D M Li, H Sun
Nov 30, 2000·Molecular and Cellular Biology·M LiK A Heidenreich
Mar 30, 2001·The Journal of Biological Chemistry·H ShengR N DuBois
Sep 21, 2001·The Biochemical Journal·S Frame, P Cohen
May 23, 2002·The Journal of Pharmacology and Experimental Therapeutics·Seung Joon BaekThomas E Eling
Jul 3, 2002·Nature Reviews. Cancer·Igor Vivanco, Charles L Sawyers
Jul 26, 2002·The Journal of Biological Chemistry·Qingding WangB Mark Evers
Mar 5, 2003·Journal of Cell Science·Bradley W Doble, James R Woodgett
May 27, 2003·International Journal of Cancer. Journal International Du Cancer·Leigh C WilsonThomas E Eling
Jun 26, 2003·The Journal of Biological Chemistry·F Gregory BuchananRaymond N DuBois
Aug 21, 2003·The Journal of Biological Chemistry·Ratan BhatJesús Avila
Oct 28, 2003·Carcinogenesis·Kianoush KhaleghpourShi-Hsiang Shen
Feb 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Desheng LuDennis A Carson
May 12, 2004·Current Cancer Drug Targets·Constantine S MitsiadesMichael Koutsilieris

❮ Previous
Next ❯

Citations

Oct 25, 2011·Cancer Metastasis Reviews·Xingya WangThomas Eling
Apr 12, 2011·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Li YangHoujie Liang
Jun 22, 2013·Cytokine & Growth Factor Reviews·Klaus UnsickerKerstin Krieglstein
Apr 17, 2008·Molecular Cancer Therapeutics·Minsub Shim, Thomas E Eling
Dec 4, 2010·Cell and Tissue Research·Katharina SchindowskiKlaus Unsicker
Apr 10, 2014·Journal of Medicinal Food·Jieun ChoiSeong-Ho Lee
Feb 6, 2007·The Veterinary Journal·Kiyoshi YamaguchiSeung Joon Baek
Aug 18, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Nam-Hui YimJin Yeul Ma
Jan 17, 2009·The Journal of Investigative Dermatology·Toshiharu YamashitaTokimasa Hida
Nov 30, 2006·Journal of Biochemistry and Molecular Biology·Thomas E ElingChang Ho Lee
Nov 21, 2009·Journal of Pharmacological Sciences·You-Jin KangSang Kook Lee
Aug 17, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Yi ZhongSeung Joon Baek
Jan 27, 2005·Biochemical and Biophysical Research Communications·Seong-Ho LeeSeung Joon Baek
Mar 26, 2011·International Journal of Cancer. Journal International Du Cancer·Mitsutoshi KadowakiThomas E Eling
Dec 4, 2010·International Journal of Cancer. Journal International Du Cancer·Preet K DhillonEdward L Giovannucci
Feb 18, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jeong-Ho KimSeung Joon Baek
Sep 22, 2010·Aging Cell·Fredrik E WiklundDavid A Brown
Jun 26, 2010·Journal of Cellular Physiology·Murielle Mimeault, Surinder K Batra
Apr 6, 2019·Current Pharmaceutical Design·Lei FangChundong Gu
Nov 6, 2013·Stem Cells Translational Medicine·Jill CorrePhilippe Bourin
Oct 27, 2015·Oncotarget·Chen LiMaode Lai
Mar 11, 2011·Journal of Cellular Physiology·Yung-Luen YuYi-Lin Sophia Chen
Mar 18, 2011·Experimental Dermatology·Andreas MauererThomas Vogt
Nov 7, 2019·Growth Factors·Anupama ModiSanjeev Misra
Apr 23, 2009·International Journal of Cancer. Journal International Du Cancer·Kathy K W AuyeungJoshua K S Ko
Apr 5, 2005·The Journal of Pharmacology and Experimental Therapeutics·Basil Rigas, Khosrow Kashfi

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis