Identification of nontuberculous mycobacteria by partial gene sequencing and public databases

International Journal of Mycobacteriology
Ines JoaoLuisa Jordao

Abstract

Nontuberculous mycobacteria (NTM) are a heterogeneous group of microorganisms with distinct clinical relevance. The treatment of NTM infections depends significantly upon the crucial identification of species at this level. The steady increase of mycobacteria species, the use of time-consuming techniques and the lack of standardized identification methods makes the achievement of this goal a demanding challenge. Additionally, inaccurate diagnosis can lead to therapeutic approaches consistent with Mycobacterium tuberculosis infection that are useless. In the present study, the performance of public databases in the accurate identification of NTM by sequence analysis of 16S rRNA and hsp65 genes were evaluated and compared. An algorithm is proposed to achieve an accurate classification of NTM in the geographic region of Portugal (Western Europe). Partial sequencing of 16S rRNA and hsp65 genes of 22 reference strains and 54 clinical isolates was performed. The resulting sequences were analysed by public web databases since their performance is evaluated statistically. The phenotypic characteristics of the isolates were also evaluated. The use of commercial kits allowed the accurate identification of 57.4% of the clinical isolates. ...Continue Reading

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Citations

Dec 17, 2015·Transboundary and Emerging Diseases·P N Kabongo-KayokaL J McGaw
Jul 17, 2015·BioMed Research International·Ali Akbar VelayatiMehdi Mirsaeidi
Mar 9, 2018·International Journal of Mycobacteriology·Claudia Andrea TortoneDelia Susana Oriani
Nov 25, 2017·International Journal of Mycobacteriology·Rog Rio RuasAntónio Sarmento
Aug 4, 2018·Journal of Medical Microbiology·Mariel Domínguez-ZepahuaRubén López-Revilla

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