Identification of novel bovine cumulus cell molecular markers predictive of oocyte competence: functional and diagnostic implications

Biology of Reproduction
Anilkumar BettegowdaGeorge W Smith

Abstract

The present study was undertaken to discover molecular markers in bovine cumulus cells predictive of oocyte competence and to elucidate their functional significance. Differences in RNA transcript abundance in cumulus cells harvested from oocytes of adult versus prepubertal animals (a model of poor oocyte quality) were identified by microarray analysis. Four genes of interest encoding for the lysosomal cysteine proteinases cathepsins B, S, K, and Z and displaying greater transcript abundance in cumulus cells surrounding oocytes harvested from prepubertal animals were chosen for further investigation. Greater mRNA abundance for such genes in cumulus cells of prepubertal oocytes was confirmed by real-time RT-PCR. Elevated transcript abundance for cathepsins B, S, and Z also was observed in cumulus cells surrounding adult metaphase II oocytes that developed to the blastocyst stage at a low percentage following parthenogenetic activation versus those that developed at a high percentage. Functional significance of cumulus cell cathepsin expression to oocyte competence was confirmed by treatment of cumulus-oocyte complexes during in vitro oocyte maturation with a cell-permeable cysteine proteinase (cathepsin) inhibitor. Inhibitor tre...Continue Reading

Associated Datasets

May 25, 2010·George W SmithAnilkumar Bettegowda

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Citations

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Datasets Mentioned

BETA
GPL325

Methods Mentioned

BETA
PCR
confocal microscopy

Software Mentioned

Expression Analysis Systematic Explorer ( EASE )
EASE
Goanna
SAS
Primer Express
Significance Analysis of Microarrays
Agbase

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