Identification of novel cyclic nucleotide binding proteins in Trypanosoma cruzi.

Molecular and Biochemical Parasitology
Adriana V JägerMartin M Edreira

Abstract

Cyclic AMP has been implicated as second messenger in a wide range of cellular processes. In the protozoan parasite Trypanosoma cruzi, cAMP is involved in the development of the parasite's life cycle. While cAMP effectors have been widely studied in other eukaryotic cells, little is known about cAMP's mechanism of action in T. cruzi. To date, only a cAMP-dependent protein kinase A (PKA) has been cloned and characterised in this parasite; however experimental evidence indicates the existence of cAMP-dependent, PKA-independent events. In order to identify new cAMP binding proteins as potential cAMP effectors, we carried out in silico studies using the predicted T. cruzi proteome. Using a combination of search methods 27 proteins with putative cNMP binding domains (CBDs) were identified. Phylogenetic analysis of the CBDs presented a homogeneous distribution, with sequences segregated into two main branches: one containing kinases-like proteins and the other gathering hypothetical proteins with different function or no other known. Comparative modelling of the strongest candidates provides support for the hypothesis that these proteins may give rise to structurally viable cyclic nucleotide binding domains. Pull-down and nucleotide ...Continue Reading

References

Aug 1, 1988·Experimental Parasitology·M Gonzales-PerdomoS Goldenberg
Jan 2, 1987·Molecular and Biochemical Parasitology·R Rangel-AldaoM Piras
Apr 1, 1984·Molecular and Biochemical Parasitology·M M OliveiraF G De Mello
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D FraidenraichM M Flawia
Dec 18, 1998·Science·H KawasakiA M Graybiel
Dec 6, 2001·Journal of Molecular Evolution·Jaume M Canaves, Susan S Taylor
Sep 11, 2004·Current Molecular Medicine·T SeebeckA Johner
Dec 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Helen M BermanSusan S Taylor
Jun 28, 2005·Nucleic Acids Research·Johannes SödingAndrei N Lupas
Jul 16, 2005·Science·Najib M El-SayedBjörn Andersson
Jul 4, 2006·Molecular and Biochemical Parasitology·Huan HuangYi Bao
Sep 11, 2007·Molecular Interventions·Sunil Laxman, Joseph A Beavo
Oct 30, 2007·Protein Science : a Publication of the Protein Society·Fred P DavisAndrej Sali
Dec 14, 2007·Genome Biology·Natarajan KannanSusan S Taylor
Aug 13, 2008·Infection and Immunity·Yi BaoHuan Huang
Apr 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Nataliya PopovychCharalampos G Kalodimos
Nov 26, 2009·Acta Tropica·Gabriel A Schmunis, Zaida E Yadon
Jan 13, 2011·FEMS Microbiology Reviews·Matthew K Gould, Harry P de Koning
Feb 1, 2012·The Journal of Infectious Diseases·Harry P de KoningThomas Seebeck
May 27, 2014·Expert Opinion on Investigational Drugs·Maria Terezinha BahiaVanessa Carla Furtado Mosqueira

❮ Previous
Next ❯

Citations

Oct 7, 2015·Frontiers in Pharmacology·Daniel N A TagoeHarry P de Koning
Mar 31, 2019·Nature Communications·Sabine BachmaierMichael Boshart
Jun 21, 2019·Current Medicinal Chemistry·Alejandra Cecilia SchoijetGuillermo Daniel Alonso
Oct 10, 2020·Molecular Microbiology·Noelia LanderRoberto Docampo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved