Identification of novel cytokine-induced genes in pancreatic beta-cells by high-density oligonucleotide arrays

Diabetes
A K CardozoD L Eizirik

Abstract

Type 1 diabetes is an autoimmune disease resulting from the selective destruction of insulin-producing beta-cells. Cytokines may contribute to pancreatic beta-cell death in type 1 diabetes. beta-cell exposure to interleukin (IL)-1beta induces functional impairment, whereas beta-cell culture for 6-9 days in the presence of IL-1beta and interferon (INF)-gamma leads to apoptosis. To clarify the mechanisms involved in these effects of cytokines, we studied the general pattern of cytokine-induced gene expression in beta-cells. Primary rat beta-cells were fluorescence-activated cell sorter-purified and exposed for 6 or 24 h to control condition, IL-1beta + INF-gamma, or IL-1beta alone (24 h only). Gene expression profile was analyzed in duplicate by oligonucleotide arrays. Nearly 3,000 transcripts were detected in controls and cytokine-treated beta-cells. Of these, 96 and 147 displayed changes in expression after 6 and 24 h, respectively, of exposure to IL-1beta + INF-gamma, whereas 105 transcripts were modified after a 24-h exposure to IL-1beta. The cytokine-responsive genes were clustered according to their biological functions. The major clusters observed were metabolism, signal transduction, transcription factors, protein synthes...Continue Reading

References

Oct 1, 1992·Diabetes/metabolism Reviews·D Pipeleers, Z Ling
Sep 1, 1985·Endocrinology·D G PipeleersW Gepts
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Mar 1, 1995·The Journal of Cell Biology·K SadoulP A Halban
Aug 1, 1994·Current Opinion in Cell Biology·S R Pfeffer
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·R J Grumont, S Gerondakis
Sep 1, 1996·Diabetologia·T Mandrup-Poulsen
Aug 1, 1997·Cytokine·J M CunninghamI C Green
Mar 24, 1998·Diabetes/metabolism Reviews·D L Eizirik, D Pavlovic
Dec 4, 1998·Diabetes·H Edlund
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·S D DerR H Silverman
Feb 4, 1999·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·J H NielsenN Billestrup
Aug 27, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S Bulfone-PausH Krause
Oct 19, 1999·The Journal of Experimental Medicine·S T GreyC Ferran
Feb 1, 2000·Trends in Endocrinology and Metabolism : TEM·T W KayJ Allison
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·G C WebbD F Steiner
Jun 14, 2000·Current Opinion in Neurobiology·A T Brunger
Jun 24, 2000·Nature·D J Lockhart, E A Winzeler
Sep 7, 2000·The New England Journal of Medicine·J Klein, A Sato
Oct 12, 2000·Journal of Neuroimmunology·B C KieseierH P Hartung

❮ Previous
Next ❯

Citations

Apr 8, 2003·The Journal of Biological Chemistry·Purevsuren JambalSubbiah Pugazhenthi
Dec 18, 2003·Annals of the New York Academy of Sciences·Thomas Mandrup-Poulsen
Jan 30, 2004·Diabetes·Sarah E EckenrodeJin-Xiong She
Dec 18, 2003·Annals of the New York Academy of Sciences·Decio L EizirikAlessandra K Cardozo
May 25, 2005·Cell and Tissue Research·Nils E MagnussonMeredin Stoltenberg
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Clayton E MathewsAlex Rabinovitch
Dec 20, 2005·Expert Review of Molecular Diagnostics·Lut OverberghChantal Mathieu
Jan 7, 2006·Diabetes/metabolism Research and Reviews·Cillian McCabeTimothy O'Brien
Oct 29, 2010·The Journal of Biological Chemistry·Fabrice MooreDecio L Eizirik
Jan 14, 2012·Cellular and Molecular Life Sciences : CMLS·Nuria C Bramswig, Klaus H Kaestner
Dec 14, 2012·PloS One·Bryan R TennantBrad G Hoffman
Mar 13, 2014·Transplant International : Official Journal of the European Society for Organ Transplantation·Céline GleizesFlorence Toti
Aug 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·Noemí Alejandra Saavedra-ÁvilaConchi Mora
Feb 3, 2015·Proteomics. Clinical Applications·Inne CrèvecoeurLut Overbergh
Nov 10, 2017·Current Opinion in Organ Transplantation·Nathan W Zammit, Shane T Grey
Sep 14, 2019·Phytotherapy Research : PTR·Altaf Al-RomaiyanPeter Jones
Dec 18, 2001·Diabetes/metabolism Research and Reviews·B NazlielB Koçer
Dec 20, 2003·Cell Death and Differentiation·S Oyadomari, M Mori

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Diabetes Association Journals

Discover the latest diabetes research published by the journals from the American Diabetes Association.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis