PMID: 9182826Jun 1, 1997Paper

Identification of novel glucocorticoid-response elements in human elastin promoter and demonstration of nucleotide sequence specificity of the receptor binding

The Journal of Investigative Dermatology
M Del MonacoJ Uitto

Abstract

Glucocorticoids exert their action on gene expression through activation of cytoplasmic glucocorticoid receptors (GRs) that bind to glucocorticoid response elements (GREs). The consensus GRE consists of two half sites (underlined), AGAACANNNTGTTCT. We have recently cloned the entire human elastin gene. Nucleotide sequencing of the promoter region disclosed the presence of three putative GREs with the downstream half-site sequence TGTTCC that has homology with the consensus GRE, although the upstream half site showed no homology. To examine the functionality of these putative GREs in binding to the GRs, we performed gel mobility shift and supershift assays with synthetic oligomers containing the putative GREs and a recombinant GR protein, expressed in a baculovirus system. All three GREs identified in the elastin promoter bound the receptor. A chimeric oligonucleotide containing the upstream consensus GRE half site and the downstream elastin promoter GRE half site was capable of binding the receptor, and this binding could be competed with the elastin promoter GRE. Nonconservative substitution of single nucleotides (positions 1-6) in the elastin GRE indicated that mutations in the positions 1-3 and 6 had relatively little effect...Continue Reading

References

Jan 1, 1991·Annals of the New York Academy of Sciences·J RosenbloomJ Uitto
Feb 10, 1989·Cell·M Beato
Jan 1, 1985·Annual Review of Pharmacology and Toxicology·G M Ringold
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Sep 15, 1994·Annals of the New York Academy of Sciences·E P SlaterM Beato

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Citations

Jun 25, 2009·Pharmacological Reviews·Hannu JärveläinenRisto Penttinen
May 27, 2015·Journal of Developmental Origins of Health and Disease·O KhorramW J Pearce
Nov 22, 2018·Cellular and Molecular Life Sciences : CMLS·Matthew W EllisYibing Qyang
Jun 7, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rasoul Alikhani-KoupaeiFelix J Frey
Jan 19, 2007·Journal of Virology·Jennifer A MertzJaquelin P Dudley
Mar 29, 2006·Hormone Research·Sabine E HannemaIeuan A Hughes
Feb 10, 1999·The American Journal of Physiology·S ChandrasekharS F Abcouwer
May 13, 2006·Experimental Dermatology·Stefanie SchoepeKhusru Asadullah
May 9, 2019·Nature Communications·Nicole Y SourenJörn Walter
Sep 19, 2019·Molecular Biology Reports·Sepideh Zununi VahedMohammadreza Ardalan
Aug 7, 1999·Progress in Retinal and Eye Research·R J Wordinger, A F Clark

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