Identification of novel HIV-1-derived HLA-E-binding peptides

Immunology Letters
Zara HannounTomáš Hanke

Abstract

Non-classical class Ib MHC-E molecule is becoming an increasingly interesting component of the immune response. It is involved in both the adaptive and innate immune responses to several chronic infections including HIV-1 and, under very specific circumstances, likely mediated a unique vaccine protection of rhesus macaques against pathogenic SIV challenge. Despite being recently in the spotlight for HIV-1 vaccine development, to date there is only one reported human leukocyte antigen (HLA)-E-binding peptide derived from HIV-1. In an effort to help start understanding the possible functions of HLA-E in HIV-1 infection, we determined novel HLA-E binding peptides derived from HIV-1 Gag, Pol and Vif proteins. These peptides were identified in three independent assays, all quantifying cell-surface stabilization of HLA-E*01:01 or HLA-E*01:03 molecules upon peptide binding, which was detected by HLA-E-specific monoclonal antibody and flow cytometry. Thus, following initial screen of over 400 HIV-1-derived 15-mer peptides, 4 novel 9-mer peptides PM9, RL9, RV9 and TP9 derived from 15-mer binders specifically stabilized surface expression of HLA-E*01:03 on the cell surface in two separate assays and 5 other binding candidates EI9, MD9, N...Continue Reading

Citations

Aug 4, 2019·Journal of Virology·Thomas van Stigt ThansAngelique Hölzemer
Oct 7, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paula RuibalSimone A Joosten
Nov 13, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sigrid D'haeseJoeri L Aerts
Mar 1, 2020·Molecular Immunology·Emma J GrantStephanie Gras
Jul 7, 2021·The Journal of Clinical Investigation·Anju BansalPaul A Goepfert

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Methods Mentioned

BETA
peptide exchange
genotyping
FCS
transfection
flow cytometry

Software Mentioned

TreeStar
FlowJo

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