Identification of novel intergenic repetitive units in a mycobacterial two-component system operon

Molecular Microbiology
P SupplyC Locht

Abstract

Mycobacterial interspersed repetitive units (MIRUs), a novel class of repeated sequences, were identified within the intercistronic region of an operon coding for a mycobacterial two-component system, named senX3-regX3. Southern blot analysis and homology searches revealed the presence of several homologous sequences in intergenic regions dispersed throughout the genomes of Mycobacterium bovis BCG, Mycobacterium tuberculosis and Mycobacterium leprae. These could be grouped into three major families, containing elements of 77-101 bp, 46-53 bp and 58-101 bp. Based on the available mycobacterial sequences, the total number of MIRUs is estimated to be about 40-50 per genome. Similar to previously identified small repetitive sequences, the MIRUs of the two-component operon are transcribed on a polycistronic mRNA. Unlike previously identified small repetitive sequences, however, MIRUs do not contain dyad symmetries, comprise small open reading frames (ORFs) whose extremities overlap those of the contiguous ORFs and are oriented in the same translational direction as those of the adjacent genes. Analyses of the sequences at the insertion sites suggest that MIRUs disseminate by transposition into DTGA sites involved in translational co...Continue Reading

Citations

Feb 15, 2000·Research in Microbiology·D A Rowe-MagnusD Mazel
Sep 13, 2001·Trends in Microbiology·R BroschS T Cole
Jun 18, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Christophe SolaNalin Rastogi
Jul 17, 2001·Molecular Microbiology·E PérezC Martín
Apr 15, 2010·Avian Pathology : Journal of the W.V.P.A·Javier MillánAlicia Aranaz
Oct 9, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Caroline AllixMaryse Fauville-Dufaux
Dec 4, 2012·Vector Borne and Zoonotic Diseases·Vivianne Cambuí Figueiredo RochaJosé Soares Ferreira Neto
Aug 2, 2011·Genome Biology and Evolution·Nicholas Delihas
Jan 11, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·I C ShamputaF Portaels
Sep 19, 2006·Journal of Bacteriology·Pablo FernandezPedro M Alzari
Jun 13, 2000·Journal of Bacteriology·T C Zahrt, V Deretic
Feb 4, 2003·Journal of Bacteriology·Aurelio Moraleda-MuñozJosé Muñoz-Dorado
Aug 6, 2010·Journal of Clinical Microbiology·Sahal A M Al-HajojAdri G M van der Zanden
Nov 6, 2004·Journal of Clinical Microbiology·Pieter OverduinDick van Soolingen
Feb 10, 2004·Journal of Clinical Microbiology·Sayera BanuRoland Brosch
Mar 9, 2004·Journal of Clinical Microbiology·Madalene RichardsonRobin M Warren
Oct 7, 2005·Journal of Clinical Microbiology·Nat SmittipatPrasit Palittapongarnpim
Nov 8, 2005·Journal of Clinical Microbiology·Kristin KremerDick van Soolingen
Feb 8, 2005·Journal of Clinical Microbiology·Z MajedM Picardeau

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.