Identification of novel Lck-derived T helper epitope long peptides applicable for HLA-A2(+) cancer patients as cancer vaccine

Cancer Science
S MatsuedaKyogo Itoh

Abstract

The present study attempted to identify T helper epitope long peptides capable of inducing cytotoxic T lymphocytes (CTL) from Lck antigen (p56(Lck) ), the src family tyrosine kinase, which is known to be aberrantly expressed in metastatic cancers cells, in order to develop a long peptide-based cancer vaccine for HLA-A2(+) cancer patients. Based on the biding motif to the HLA-DR and HLA-A2 alleles, 94 peptides were prepared from the Lck antigen. These peptides were screened for their reactivity to immunoglobulin G (IgG) from plasma of cancer patients, followed by testing of their ability to induce both CD4(+) and CD8(+) T lymphocytes showing not only peptide-specific IFN-γ production but cytotoxicity against HLA-A2(+) cancer cells from peripheral blood mononuclear cells (PBMC) of HLA-A2(+) cancer patients. Among 94 peptides tested, the three T helper epitope long peptides and their inner CTL epitope short peptides with HLA-A2 binding motifs were frequently recognized by IgG of cancer patients, and efficiently induced both CD4(+) IFN-γ(+) and CD8(+) IFN-γ(+) T lymphocytes. Patients' PBMC stimulated with these long peptides showed cytotoxicity against HLA-A2(+) Lck(+) cancer cells in HLA-class I and HLA-class II dependent manners....Continue Reading

References

May 16, 2002·Cancer Immunology, Immunotherapy : CII·Naoya HidaKyogo Itoh
Feb 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mamoru HaradaKyogo Itoh
Oct 6, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Satoko MatsuedaMamoru Harada
Apr 18, 2008·Nature Reviews. Cancer·Cornelis J M Melief, Sjoerd H van der Burg
Sep 3, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Martin A CheeverLynn M Matrisian
Jun 8, 2010·The New England Journal of Medicine·F Stephen HodiWalter J Urba
Dec 15, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Mizuhiko TerasakiKyogo Itoh
Jun 5, 2012·The New England Journal of Medicine·Suzanne L TopalianMario Sznol
Dec 1, 2012·Cancer Immunology, Immunotherapy : CII·Masanori NoguchiKyogo Itoh
Feb 5, 2013·Nature·Heidi BraumüllerMartin Röcken
May 30, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yusuke TomitaYasuharu Nishimura
Feb 15, 2014·Current Medicinal Chemistry·Tetsuro SasadaKyogo Itoh
May 2, 2014·Frontiers in Immunology·Ole Audun Werner HaabethBjarne Bogen

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
flow cytometry

Software Mentioned

MULTIPRED
EpiTOP SYFPEIT

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.