Identification of novel low-dose bisphenol a targets in human foreskin fibroblast cells derived from hypospadias patients.

PloS One
Xian-Yang QinHideko Sone

Abstract

The effect of low-dose bisphenol A (BPA) exposure on human reproductive health is still controversial. To better understand the molecular basis of the effect of BPA on human reproductive health, a genome-wide screen was performed using human foreskin fibroblast cells (hFFCs) derived from child hypospadias (HS) patients to identify novel targets of low-dose BPA exposure. Gene expression profiles of hFFCs were measured after exposure to 10 nM BPA, 0.01 nM 17β-estradiol (E2) or 1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) for 24 h. Differentially expressed genes were identified using an unpaired Student's t test with P value cut off at 0.05 and fold change of more than 1.2. These genes were selected for network generation and pathway analysis using Ingenuity Pathways Analysis, Pathway Express and KegArray. Seventy-one genes (42 downregulated and 29 upregulated) were identified as significantly differentially expressed in response to BPA, among which 43 genes were found to be affected exclusively by BPA compared with E2 and TCDD. Of particular interest, real-time PCR analysis revealed that the expression of matrix metallopeptidase 11 (MMP11), a well-known effector of development and normal physiology, was found to be inhibited b...Continue Reading

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Citations

Nov 26, 2015·Reproductive Toxicology·Mariana F FernándezNicolás Olea
May 9, 2014·Journal of Cellular Physiology·Nuria Olea-HerreroRicardo J Bosch
Nov 26, 2015·Molecular BioSystems·Jack Yu-Shih LinQing-Dong Ling
Apr 9, 2015·Journal of Cancer Prevention·Oneyeol YangYoung Rok Seo

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Methods Mentioned

BETA
Feature Extraction
chemical treatments
PCR
PCA
genetic interference

Software Mentioned

Ingenuity Pathways Analysis ( IPA )
GeneSpring GX
SIMCA
IPA
Sequence Detection System
GeneSpring
P +
Ingenuity Pathways Knowledge Base
KegArray
Pathway Express

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