Identification of novel noncoding transcripts in telomerase-negative yeast using RNA-seq

Scientific Reports
Rachel O NiedererDavid C Zappulla

Abstract

Telomerase is a ribonucleoprotein that maintains the ends of linear chromosomes in most eukaryotes. Loss of telomerase activity results in shortening of telomeric DNA and eventually a specific G2/M cell-cycle arrest known as senescence. In humans, telomere shortening occurs during aging, while inappropriate activation of telomerase is associated with approximately 90% of cancers. Previous studies have identified several classes of noncoding RNAs (ncRNA) also associated with aging-related senescence and cancer, but whether ncRNAs are also involved in short-telomere-induced senescence in yeast is unknown. Here, we report 112 putative novel lncRNAs in the yeast Saccharomyces cerevisiae, 41 of which are only expressed in telomerase-negative yeast. Expression of approximately half of the lncRNAs is strongly correlated with that of adjacent genes, suggesting this subset may influence transcription of neighboring genes. Our results reveal a new potential mechanism governing adaptive changes in senescing and post-senescent survivor yeast cells.

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Citations

Jul 16, 2019·Yeast·Zhou Xu, Maria Teresa Teixeira
Dec 22, 2016·Frontiers in Endocrinology·Ufuk Degirmenci, Sun Lei
Dec 18, 2021·Nature Communications·Hrant Hovhannisyan, Toni Gabaldón

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Methods Mentioned

BETA
dissection
RNA-seq

Software Mentioned

Coding - Potential Calculator ( CPC
Tophat
Cufflinks
R Studio
Bowtie2Index
RiboZero
Database for Annotation , Visualization and Integrated Discove...
FastQC
MiSeq
CASAVA

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