Identification of Novel Protein Lysine Acetyltransferases in Escherichia coli

MBio
David G ChristensenAlan J Wolfe

Abstract

Posttranslational modifications, such as Nε-lysine acetylation, regulate protein function. Nε-lysine acetylation can occur either nonenzymatically or enzymatically. The nonenzymatic mechanism uses acetyl phosphate (AcP) or acetyl coenzyme A (AcCoA) as acetyl donor to modify an Nε-lysine residue of a protein. The enzymatic mechanism uses Nε-lysine acetyltransferases (KATs) to specifically transfer an acetyl group from AcCoA to Nε-lysine residues on proteins. To date, only one KAT (YfiQ, also known as Pka and PatZ) has been identified in Escherichia coli Here, we demonstrate the existence of 4 additional E. coli KATs: RimI, YiaC, YjaB, and PhnO. In a genetic background devoid of all known acetylation mechanisms (most notably AcP and YfiQ) and one deacetylase (CobB), overexpression of these putative KATs elicited unique patterns of protein acetylation. We mutated key active site residues and found that most of them eliminated enzymatic acetylation activity. We used mass spectrometry to identify and quantify the specificity of YfiQ and the four novel KATs. Surprisingly, our analysis revealed a high degree of substrate specificity. The overlap between KAT-dependent and AcP-dependent acetylation was extremely limited, supporting the ...Continue Reading

References

May 20, 1990·European Journal of Biochemistry·M C Serre, J R Garel
Jun 20, 1989·Journal of Molecular Biology·W R Rypniewski, P R Evans
Jan 27, 1999·Bioinformatics·S R Eddy
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·Kirill A Datsenko, Barry L Wanner
Jul 14, 2000·Journal of Bacteriology·S KumariAlan J Wolfe
May 20, 2003·Molecular Microbiology·Alan J WolfeTyrrell Conway
Jul 9, 2004·Journal of Molecular Biology·Vincent J Starai, Jorge C Escalante-Semerena
Dec 8, 2004·Archives of Biochemistry and Biophysics·Matthew W VettingJohn S Blanchard
Mar 1, 2006·Biochemistry·James C Errey, John S Blanchard
Jun 14, 2006·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Masanari KitagawaHirotada Mori
Jun 20, 2006·The Journal of Biological Chemistry·Birgit SchillingLisa M Ellerby
Oct 3, 2006·The Journal of Biological Chemistry·Salma RafiPeter J Tonge
Jan 16, 2007·Environmental Microbiology·Joanna DomkaThomas K Wood
Nov 6, 2007·Biotechnology and Bioengineering·Akinobu ItoSatoshi Okabe
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Mar 17, 2009·Biotechnology and Bioengineering·Akinobu ItoSatoshi Okabe
Apr 21, 2009·Applied and Environmental Microbiology·Akinobu ItoSatoshi Okabe
Nov 3, 2009·Nature Biotechnology·Byung-Kwan ChoBernhard Ø Palsson
Feb 2, 2010·Journal of Proteome Research·Danielle L SwaneyJoshua J Coon
May 13, 2010·Molecular Systems Biology·Szymon JozefczukLothar Willmitzer
Oct 5, 2010·Archaea : an International Microbiological Journal·Jörg Soppa
Feb 26, 2011·The Journal of Biological Chemistry·Louiza ZerradMatthew W Bowler
Jul 9, 2013·Molecular Cell·Brian T WeinertChunaram Choudhary
Jul 16, 2013·Journal of Bacteriology·Linda I HuAlan J Wolfe
Jan 16, 2014·Molecular & Cellular Proteomics : MCP·Jesse G MeyerElizabeth A Komives
Apr 23, 2014·Nucleic Acids Research·Xavier Robert, Patrice Gouet
Nov 2, 2014·Nature Communications·Sangtae Kim, Pavel A Pevzner
Dec 18, 2014·Molecular Systems Biology·Sara Castaño-CerezoManuel Cánovas
Jan 20, 2015·Nature Methods·Chih-Chiang TsouAlexey I Nesvizhskii
Jan 30, 2015·Proteomics. Clinical Applications·Eric W DeutschRobert L Moritz
Apr 1, 2015·Nucleic Acids Research·T TatusovaI Tolstoy
May 8, 2015·Nature Protocols·Lawrence A KelleyMichael J E Sternberg
Jul 17, 2015·Microbiology and Molecular Biology Reviews : MMBR·Kristy L Hentchel, Jorge C Escalante-Semerena
Aug 8, 2015·The Journal of Biological Chemistry·Teresa de Diego PuenteManuel Cánovas Díaz
Aug 13, 2015·Molecular Microbiology·Birgit SchillingAlan J Wolfe
Jan 29, 2016·Biochemistry·Lorenza FavrotOlivia Vergnolle
Feb 6, 2016·Nucleic Acids Research·Limin SongWenxing Liang
Jun 15, 2016·Biochimica Et Biophysica Acta·Adrian DrazicThomas Arnesen
Apr 4, 2017·Journal of Molecular Biology·Sumana VenkatChenguang Fan
Jul 26, 2017·Chembiochem : a European Journal of Chemical Biology·Sumana VenkatChenguang Fan

Citations

Apr 12, 2019·The FEBS Journal·Sumana VenkatChenguang Fan
Sep 28, 2019·Current Genetics·Ewa Laskowska, Dorota Kuczyńska-Wiśnik
Jul 8, 2020·Molecular Systems Biology·Willy V BienvenutIris Finkemeier
May 16, 2019·Annual Review of Microbiology·Chelsey M VanDrisse, Jorge C Escalante-Semerena
Feb 7, 2020·International Journal of Molecular Sciences·Kangsan KimByung-Kwan Cho
Apr 17, 2020·Microbiology and Molecular Biology Reviews : MMBR·Rachel M Burckhardt, Jorge C Escalante-Semerena
Apr 11, 2019·MBio·David G ChristensenAlan J Wolfe
Nov 20, 2019·Nature Chemical Biology·Zhenrun J ZhangHoward C Hang
Feb 11, 2021·The Journal of Microbiology·Jaejin LeeKangseok Lee
Jun 13, 2019·Biological Chemistry·Jürgen LassakWolfram Volkwein
Dec 16, 2020·Infection and Immunity·Jianhui LiYu-Feng Yao
Mar 30, 2021·The Journal of Microbiology·Jaejin LeeKangseok Lee
Apr 8, 2021·Protein Science : a Publication of the Protein Society·Van Thi Bich LeMisty L Kuhn

Datasets Mentioned

BETA
MSV000082411
PXD009940

Methods Mentioned

BETA
acetylation
phosphotransferase
electrophoresis
acetylations
NMR
motility assay
lipoylation
acetylate
affinity purification
chip

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